Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment

• Main Authors: Rodin, S, Antonsson, L, Niaudet, C, Simonson, O.E, Salmela, E, Hansson, E.M, Domogatskaya, A, Xiao, Z, Damdimopoulou, P, Sheikhi, M, Inzunza, J, Nilsson, A.-S, Baker, D, Kuiper, R, Sun, Y, Blennow, E, Nordenskjöld, M, Grinnemo, K.-H, Kere, J, Betsholtz, C, Hovatta, O, Tryggvason, K
• Other Authors: DUKE-NUS MEDICAL SCHOOL
• Format: Article
• Published: 2020
• Subjects: laminin; laminin 521; unclassified drug; uvomorulin; cadherin; laminin 11 protein, human; phosphatidylinositol 3 kinase; protein kinase B; very late activation antigen 6; biotechnology; embryo; inhibitor; phenotype; protein; recombination; article; blastoma; cell cloning; cell renewal; cell survival; controlled study; embryonic stem cell; human; human cell; inner cell mass; stem cell niche; xeno-free culture; culture technique; karyotyping; metabolism; physiology; Cadherins; Cell Culture Techniques; Embryonic Stem Cells; Humans; Integrin alpha6beta1; Karyotyping; Laminin; Phosphatidylinositol 3-Kinases; Proto-Oncogene Proteins c-akt
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/177772
• Institution: National University of Singapore

10.1038/ncomms4195