Rapid production of pure recombinant actin isoforms in Pichia pastoris

• Main Authors: Hatano, T, Alioto, S, Roscioli, E, Palani, S, Clarke, S.T, Kamnev, A, Hernandez-Fernaud, J.R, Sivashanmugam, L, Chapa-y-Lazo, B, Jones, A.M.E, Robinson, R.C, Sampath, K, Mishima, M, McAinsh, A.D, Goode, B.L, Balasubramanian, M.K
• Other Authors: CHEMICAL & BIOMOLECULAR ENGINEERING
• Format: Article
• Published: Company of Biologists Ltd 2020
• Subjects: actin; isoprotein; recombinant protein; thymosin beta4; Article; binding affinity; controlled study; cytoskeleton; dendrite; heterologous expression; in vitro study; in vivo study; Komagataella pastoris; molecular biology; nonhuman; priority journal; protein expression; protein modification; protein purification; protein synthesis; Saccharomyces cerevisiae; Schizosaccharomyces pombe; animal; human; metabolism; Pichia; Actins; Animals; Humans; Protein Isoforms
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/179043
• Institution: National University of Singapore

10.1242/jcs.213827