Chondroitin sulphate (WF6 epitope) levels in peri-miniscrew implant crevicular fluid during orthodontic loading

The aim of this study was to monitor changes in chondroitin sulphate (CS; WF6 epitope) levels in peri-miniscrew implant crevicular fluid (PMICF) during orthodontic loading.Ten patients (seven males and three females; aged 22.0 ± 3.4 years), who required orthodontic treatment with extraction of all f...

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Main Authors: Intachai I., Krisanaprakornkit S., Kongtawelert P., Ong-Chai S., Buranastidporn B., Suzuki E.Y., Jotikasthira D.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-77951638995&partnerID=40&md5=3000c91182d638b3bd4a150b0f0ce53d
http://www.ncbi.nlm.nih.gov/pubmed/19752017
http://cmuir.cmu.ac.th/handle/6653943832/1043
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-10432014-08-29T09:17:39Z Chondroitin sulphate (WF6 epitope) levels in peri-miniscrew implant crevicular fluid during orthodontic loading Intachai I. Krisanaprakornkit S. Kongtawelert P. Ong-Chai S. Buranastidporn B. Suzuki E.Y. Jotikasthira D. The aim of this study was to monitor changes in chondroitin sulphate (CS; WF6 epitope) levels in peri-miniscrew implant crevicular fluid (PMICF) during orthodontic loading.Ten patients (seven males and three females; aged 22.0 ± 3.4 years), who required orthodontic treatment with extraction of all four premolar teeth, participated in the study. Twenty miniscrew implants (used as orthodontic anchorage) were placed, two in each patient, buccally and bilaterally in the alveolar bone between the roots of the maxillary posterior teeth. Sentalloy closed-coil springs (50 g) were used to load the miniscrew implants and to move the maxillary canines distally. During the unloaded period, PMICF samples were collected on days 1, 3, 5, and 7 after miniscrew implant placement and on days 14, 21, 28, and 35 during the loaded period. Clinical mobility assessments of the miniscrew implants were recorded at each visit. The competitive enzyme-linked immunosorbent assay with monoclonal antibody WF6 was used to detect CS (WF6 epitope) levels in the PMICF samples. The differences between the CS (WF6 epitope) levels during the unloaded and loaded periods were determined by a Mann-Whitney U-test.During the loaded period, two miniscrew implants were considered to have failed. The CS (WF6 epitope) levels during the unloaded period ranged from 0.00 to 758.03 ng/ml and those during the loaded period from 0.00 to 1025.11 ng/ml. Medians of CS (WF6 epitope) levels, around 'immobile' miniscrew implants, between the unloaded and loaded periods were not significantly different (P=0.07).CS (WF6 epitope) levels in PMICF can be detected and may be used as biomarkers for assessing alveolar bone remodelling around miniscrew implants during orthodontic loading. © The Author 2008. Published by Oxford University Press on behalf of the European Orthodontic Society. All rights reserved. 2014-08-29T09:17:39Z 2014-08-29T09:17:39Z 2010 Article 1415387 10.1093/ejo/cjp056 19752017 EJOOD http://www.scopus.com/inward/record.url?eid=2-s2.0-77951638995&partnerID=40&md5=3000c91182d638b3bd4a150b0f0ce53d http://www.ncbi.nlm.nih.gov/pubmed/19752017 http://cmuir.cmu.ac.th/handle/6653943832/1043 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description The aim of this study was to monitor changes in chondroitin sulphate (CS; WF6 epitope) levels in peri-miniscrew implant crevicular fluid (PMICF) during orthodontic loading.Ten patients (seven males and three females; aged 22.0 ± 3.4 years), who required orthodontic treatment with extraction of all four premolar teeth, participated in the study. Twenty miniscrew implants (used as orthodontic anchorage) were placed, two in each patient, buccally and bilaterally in the alveolar bone between the roots of the maxillary posterior teeth. Sentalloy closed-coil springs (50 g) were used to load the miniscrew implants and to move the maxillary canines distally. During the unloaded period, PMICF samples were collected on days 1, 3, 5, and 7 after miniscrew implant placement and on days 14, 21, 28, and 35 during the loaded period. Clinical mobility assessments of the miniscrew implants were recorded at each visit. The competitive enzyme-linked immunosorbent assay with monoclonal antibody WF6 was used to detect CS (WF6 epitope) levels in the PMICF samples. The differences between the CS (WF6 epitope) levels during the unloaded and loaded periods were determined by a Mann-Whitney U-test.During the loaded period, two miniscrew implants were considered to have failed. The CS (WF6 epitope) levels during the unloaded period ranged from 0.00 to 758.03 ng/ml and those during the loaded period from 0.00 to 1025.11 ng/ml. Medians of CS (WF6 epitope) levels, around 'immobile' miniscrew implants, between the unloaded and loaded periods were not significantly different (P=0.07).CS (WF6 epitope) levels in PMICF can be detected and may be used as biomarkers for assessing alveolar bone remodelling around miniscrew implants during orthodontic loading. © The Author 2008. Published by Oxford University Press on behalf of the European Orthodontic Society. All rights reserved.
format Article
author Intachai I.
Krisanaprakornkit S.
Kongtawelert P.
Ong-Chai S.
Buranastidporn B.
Suzuki E.Y.
Jotikasthira D.
spellingShingle Intachai I.
Krisanaprakornkit S.
Kongtawelert P.
Ong-Chai S.
Buranastidporn B.
Suzuki E.Y.
Jotikasthira D.
Chondroitin sulphate (WF6 epitope) levels in peri-miniscrew implant crevicular fluid during orthodontic loading
author_facet Intachai I.
Krisanaprakornkit S.
Kongtawelert P.
Ong-Chai S.
Buranastidporn B.
Suzuki E.Y.
Jotikasthira D.
author_sort Intachai I.
title Chondroitin sulphate (WF6 epitope) levels in peri-miniscrew implant crevicular fluid during orthodontic loading
title_short Chondroitin sulphate (WF6 epitope) levels in peri-miniscrew implant crevicular fluid during orthodontic loading
title_full Chondroitin sulphate (WF6 epitope) levels in peri-miniscrew implant crevicular fluid during orthodontic loading
title_fullStr Chondroitin sulphate (WF6 epitope) levels in peri-miniscrew implant crevicular fluid during orthodontic loading
title_full_unstemmed Chondroitin sulphate (WF6 epitope) levels in peri-miniscrew implant crevicular fluid during orthodontic loading
title_sort chondroitin sulphate (wf6 epitope) levels in peri-miniscrew implant crevicular fluid during orthodontic loading
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-77951638995&partnerID=40&md5=3000c91182d638b3bd4a150b0f0ce53d
http://www.ncbi.nlm.nih.gov/pubmed/19752017
http://cmuir.cmu.ac.th/handle/6653943832/1043
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