Involvement of cytosolic phospholipase A2alpha in MMP-9 up-regulation

Matrix metalloproteinase-9 (MMP-9) is important in the pathogenesis of periodontitis. Cytosolic phospholipase A(2)alpha (cPLA(2)alpha) is involved in MMP-9 up-regulation in human monocytes. We tested the hypothesis that cPLA(2)alpha also regulates MMP-9 induction by Fusobacterium nucleatum and by ph...

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Main Authors: Montreekachon P., Chotjumlong P., Reutrakul V., Krisanaprakornkit S.
Format: Article
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/1087
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-10872014-08-29T09:17:44Z Involvement of cytosolic phospholipase A2alpha in MMP-9 up-regulation Montreekachon P. Chotjumlong P. Reutrakul V. Krisanaprakornkit S. Matrix metalloproteinase-9 (MMP-9) is important in the pathogenesis of periodontitis. Cytosolic phospholipase A(2)alpha (cPLA(2)alpha) is involved in MMP-9 up-regulation in human monocytes. We tested the hypothesis that cPLA(2)alpha also regulates MMP-9 induction by Fusobacterium nucleatum and by phorbol 12-myristate-13-acetate (PMA) in gingival epithelial cells. While PMA induced MMP-9 expression considerably, F. nucleatum did so moderately. This time-course study demonstrated that MMP-9 mRNA up-regulation occurred at 3 hours, whereas MMP-9 secretion and activity in cell-free supernatants occurred at 12 hours. cPLA(2)alpha mRNA was constitutively expressed in gingival epithelial cells. Transient activation of cPLA(2) by Ser505 phosphorylation was observed in the nuclei upon stimulation, suggesting its role as a transcription factor, while cPLA(2) protein expression remained unchanged. Induction of MMP-9 expression and activity was significantly inhibited by 1 muM of the specific cPLA(2)alpha inhibitor (P < 0.01). These findings demonstrate the involvement of cPLA(2)alpha in MMP-9 up-regulation. 2014-08-29T09:17:44Z 2014-08-29T09:17:44Z 2009 Journal Article 1544-0591 19828892 http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/1087 eng
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Matrix metalloproteinase-9 (MMP-9) is important in the pathogenesis of periodontitis. Cytosolic phospholipase A(2)alpha (cPLA(2)alpha) is involved in MMP-9 up-regulation in human monocytes. We tested the hypothesis that cPLA(2)alpha also regulates MMP-9 induction by Fusobacterium nucleatum and by phorbol 12-myristate-13-acetate (PMA) in gingival epithelial cells. While PMA induced MMP-9 expression considerably, F. nucleatum did so moderately. This time-course study demonstrated that MMP-9 mRNA up-regulation occurred at 3 hours, whereas MMP-9 secretion and activity in cell-free supernatants occurred at 12 hours. cPLA(2)alpha mRNA was constitutively expressed in gingival epithelial cells. Transient activation of cPLA(2) by Ser505 phosphorylation was observed in the nuclei upon stimulation, suggesting its role as a transcription factor, while cPLA(2) protein expression remained unchanged. Induction of MMP-9 expression and activity was significantly inhibited by 1 muM of the specific cPLA(2)alpha inhibitor (P < 0.01). These findings demonstrate the involvement of cPLA(2)alpha in MMP-9 up-regulation.
format Article
author Montreekachon P.
Chotjumlong P.
Reutrakul V.
Krisanaprakornkit S.
spellingShingle Montreekachon P.
Chotjumlong P.
Reutrakul V.
Krisanaprakornkit S.
Involvement of cytosolic phospholipase A2alpha in MMP-9 up-regulation
author_facet Montreekachon P.
Chotjumlong P.
Reutrakul V.
Krisanaprakornkit S.
author_sort Montreekachon P.
title Involvement of cytosolic phospholipase A2alpha in MMP-9 up-regulation
title_short Involvement of cytosolic phospholipase A2alpha in MMP-9 up-regulation
title_full Involvement of cytosolic phospholipase A2alpha in MMP-9 up-regulation
title_fullStr Involvement of cytosolic phospholipase A2alpha in MMP-9 up-regulation
title_full_unstemmed Involvement of cytosolic phospholipase A2alpha in MMP-9 up-regulation
title_sort involvement of cytosolic phospholipase a2alpha in mmp-9 up-regulation
publishDate 2014
url http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/1087
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