Aggregatibacter actinomycetemcomitans serotypes, the JP2 clone and cytolethal distending toxin genes in a Thai population

Aim To examine the genetic diversity of Aggregatibacter actinomycetemcomitans in Thai adults. Materials and Methods Subgingival plaque samples from 453 subjects were analysed for A. actinomycetemcomitans serotypes, the presence of the high leukotoxin-producing JP2 clone and cytolethal distending tox...

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Main Authors: Bandhaya P., Saraithong P., Likittanasombat K., Hengprasith B., Torrungruang K.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84861098004&partnerID=40&md5=23384ca9c064ce8d725f1e4b80a3a023
http://cmuir.cmu.ac.th/handle/6653943832/1135
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spelling th-cmuir.6653943832-11352014-08-29T09:17:47Z Aggregatibacter actinomycetemcomitans serotypes, the JP2 clone and cytolethal distending toxin genes in a Thai population Bandhaya P. Saraithong P. Likittanasombat K. Hengprasith B. Torrungruang K. Aim To examine the genetic diversity of Aggregatibacter actinomycetemcomitans in Thai adults. Materials and Methods Subgingival plaque samples from 453 subjects were analysed for A. actinomycetemcomitans serotypes, the presence of the high leukotoxin-producing JP2 clone and cytolethal distending toxin genes (cdtABC) using the polymerase chain reaction technique. In subjects who were positive for cdtABC, restriction fragment length polymorphism analysis was used to identify a single nucleotide polymorphism (SNP) in the cdtB gene at amino acid position 281. The extent and severity of periodontal disease were compared between subjects harbouring different A. actinomycetemcomitans genotypes. Results Eighty six subjects (19%) were positive for A. actinomycetemcomitans. The JP2 clone was not detected. Serotype c was the most prevalent (57%), followed by serotypes a (33%) and b (7%). Among A. actinomycetemcomitans-positive subjects, 27% were positive for cdtABC. All cdtABC-positive subjects possessed the SNP in the cdtB, which is involved with increased toxin activity. The presence of A. actinomycetemcomitans, but not a specific genotype, was significantly related to increased probing depth and periodontal attachment loss. Conclusions Our results confirm the previous findings that genotype distribution of A. actinomycetemcomitans varies between ethnic groups. However, no clear relationship between a specific genotype and periodontal conditions was observed. © 2012 John Wiley & Sons A/S. 2014-08-29T09:17:47Z 2014-08-29T09:17:47Z 2012 Article 3036979 10.1111/j.1600-051X.2012.01871.x 22471788 JCPED http://www.scopus.com/inward/record.url?eid=2-s2.0-84861098004&partnerID=40&md5=23384ca9c064ce8d725f1e4b80a3a023 http://cmuir.cmu.ac.th/handle/6653943832/1135 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Aim To examine the genetic diversity of Aggregatibacter actinomycetemcomitans in Thai adults. Materials and Methods Subgingival plaque samples from 453 subjects were analysed for A. actinomycetemcomitans serotypes, the presence of the high leukotoxin-producing JP2 clone and cytolethal distending toxin genes (cdtABC) using the polymerase chain reaction technique. In subjects who were positive for cdtABC, restriction fragment length polymorphism analysis was used to identify a single nucleotide polymorphism (SNP) in the cdtB gene at amino acid position 281. The extent and severity of periodontal disease were compared between subjects harbouring different A. actinomycetemcomitans genotypes. Results Eighty six subjects (19%) were positive for A. actinomycetemcomitans. The JP2 clone was not detected. Serotype c was the most prevalent (57%), followed by serotypes a (33%) and b (7%). Among A. actinomycetemcomitans-positive subjects, 27% were positive for cdtABC. All cdtABC-positive subjects possessed the SNP in the cdtB, which is involved with increased toxin activity. The presence of A. actinomycetemcomitans, but not a specific genotype, was significantly related to increased probing depth and periodontal attachment loss. Conclusions Our results confirm the previous findings that genotype distribution of A. actinomycetemcomitans varies between ethnic groups. However, no clear relationship between a specific genotype and periodontal conditions was observed. © 2012 John Wiley & Sons A/S.
format Article
author Bandhaya P.
Saraithong P.
Likittanasombat K.
Hengprasith B.
Torrungruang K.
spellingShingle Bandhaya P.
Saraithong P.
Likittanasombat K.
Hengprasith B.
Torrungruang K.
Aggregatibacter actinomycetemcomitans serotypes, the JP2 clone and cytolethal distending toxin genes in a Thai population
author_facet Bandhaya P.
Saraithong P.
Likittanasombat K.
Hengprasith B.
Torrungruang K.
author_sort Bandhaya P.
title Aggregatibacter actinomycetemcomitans serotypes, the JP2 clone and cytolethal distending toxin genes in a Thai population
title_short Aggregatibacter actinomycetemcomitans serotypes, the JP2 clone and cytolethal distending toxin genes in a Thai population
title_full Aggregatibacter actinomycetemcomitans serotypes, the JP2 clone and cytolethal distending toxin genes in a Thai population
title_fullStr Aggregatibacter actinomycetemcomitans serotypes, the JP2 clone and cytolethal distending toxin genes in a Thai population
title_full_unstemmed Aggregatibacter actinomycetemcomitans serotypes, the JP2 clone and cytolethal distending toxin genes in a Thai population
title_sort aggregatibacter actinomycetemcomitans serotypes, the jp2 clone and cytolethal distending toxin genes in a thai population
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84861098004&partnerID=40&md5=23384ca9c064ce8d725f1e4b80a3a023
http://cmuir.cmu.ac.th/handle/6653943832/1135
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