Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study

Introduction: A 3-antibiotic combination (3Mix) is widely used in endodontics for root canal disinfection, particularly in pulp revascularization procedures. However, the cytotoxicity of 3Mix has not been evaluated. The purpose of this study was to determine the cytotoxicity and antibacterial effica...

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Main Authors: Chuensombat S., Khemaleelakul S., Chattipakorn S., Srisuwan T.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84877875951&partnerID=40&md5=27f609775fd649d8a1929b014b7073ba
http://cmuir.cmu.ac.th/handle/6653943832/1169
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spelling th-cmuir.6653943832-11692014-08-29T09:17:50Z Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study Chuensombat S. Khemaleelakul S. Chattipakorn S. Srisuwan T. Introduction: A 3-antibiotic combination (3Mix) is widely used in endodontics for root canal disinfection, particularly in pulp revascularization procedures. However, the cytotoxicity of 3Mix has not been evaluated. The purpose of this study was to determine the cytotoxicity and antibacterial efficacy of 3Mix and each single antibiotic component of 3Mix. Methods: For the cytotoxicity test, human dental pulp cells and apical papilla cells were exposed to either 3Mix or to each single antibiotic component of 3Mix using concentrations of 0.024, 0.097, 0.39, 1.56, 6.25, and 25.00 μg/mL for 1, 3, 5, and 7 days. Cell viability was determined using the 3-[4,5-dimethylthiazol-2- yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. For the antibacterial test, 25.00 μg/mL and 0.39 μg/mL 3Mix or single antibiotic were tested on bacteria isolated from necrotic teeth by measuring bacterial recovery on blood agar. Results: The 0.024-μg/mL concentration of all experimental groups generated the highest dental pulp cell or apical pulp cell viability at all time periods. On day 7, 0.39 μg/mL 3Mix produced more than 90% cell viability; 25.00 μg/mL 3Mix completely eliminated isolated bacteria, whereas 0.39 μg/mL was unable to eradicate all bacteria. However, the overall bacterial reduction was significantly different compared with the control group (P < .01). Conclusions: All drugs except metronidazole induced cytotoxicity on cultured cells. 3Mix generated higher cytotoxicity compared with a single drug. The cytotoxicity increased in a concentration- and time-dependent manner; 0.39 μg/mL 3Mix had less cytotoxicity and was able to significantly reduce bacteria isolated from necrotic teeth. Copyright © 2013 American Association of Endodontists. 2014-08-29T09:17:50Z 2014-08-29T09:17:50Z 2013 Article 00992399 10.1016/j.joen.2012.11.041 JOEND http://www.scopus.com/inward/record.url?eid=2-s2.0-84877875951&partnerID=40&md5=27f609775fd649d8a1929b014b7073ba http://cmuir.cmu.ac.th/handle/6653943832/1169 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Introduction: A 3-antibiotic combination (3Mix) is widely used in endodontics for root canal disinfection, particularly in pulp revascularization procedures. However, the cytotoxicity of 3Mix has not been evaluated. The purpose of this study was to determine the cytotoxicity and antibacterial efficacy of 3Mix and each single antibiotic component of 3Mix. Methods: For the cytotoxicity test, human dental pulp cells and apical papilla cells were exposed to either 3Mix or to each single antibiotic component of 3Mix using concentrations of 0.024, 0.097, 0.39, 1.56, 6.25, and 25.00 μg/mL for 1, 3, 5, and 7 days. Cell viability was determined using the 3-[4,5-dimethylthiazol-2- yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. For the antibacterial test, 25.00 μg/mL and 0.39 μg/mL 3Mix or single antibiotic were tested on bacteria isolated from necrotic teeth by measuring bacterial recovery on blood agar. Results: The 0.024-μg/mL concentration of all experimental groups generated the highest dental pulp cell or apical pulp cell viability at all time periods. On day 7, 0.39 μg/mL 3Mix produced more than 90% cell viability; 25.00 μg/mL 3Mix completely eliminated isolated bacteria, whereas 0.39 μg/mL was unable to eradicate all bacteria. However, the overall bacterial reduction was significantly different compared with the control group (P < .01). Conclusions: All drugs except metronidazole induced cytotoxicity on cultured cells. 3Mix generated higher cytotoxicity compared with a single drug. The cytotoxicity increased in a concentration- and time-dependent manner; 0.39 μg/mL 3Mix had less cytotoxicity and was able to significantly reduce bacteria isolated from necrotic teeth. Copyright © 2013 American Association of Endodontists.
format Article
author Chuensombat S.
Khemaleelakul S.
Chattipakorn S.
Srisuwan T.
spellingShingle Chuensombat S.
Khemaleelakul S.
Chattipakorn S.
Srisuwan T.
Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study
author_facet Chuensombat S.
Khemaleelakul S.
Chattipakorn S.
Srisuwan T.
author_sort Chuensombat S.
title Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study
title_short Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study
title_full Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study
title_fullStr Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study
title_full_unstemmed Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study
title_sort cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: an in vitro study
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84877875951&partnerID=40&md5=27f609775fd649d8a1929b014b7073ba
http://cmuir.cmu.ac.th/handle/6653943832/1169
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