An in vitro culture system for long-term expansion of epithelial and mesenchymal salivary gland cells: Role of TGF- β 1 in salivary gland epithelial and mesenchymal differentiation
Despite a pivotal role in salivary gland development, homeostasis, and disease, the role of salivary gland mesenchyme is not well understood. In this study, we used the Col1a1-GFP mouse model to characterize the salivary gland mesenchyme in vitro and in vivo. The Col1a1-GFP transgene was exclusively...
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th-cmuir.6653943832-11712014-08-29T09:17:50Z An in vitro culture system for long-term expansion of epithelial and mesenchymal salivary gland cells: Role of TGF- β 1 in salivary gland epithelial and mesenchymal differentiation Janebodin K. Buranaphatthana W. Ieronimakis N. Hays A.L. Reyes M. Despite a pivotal role in salivary gland development, homeostasis, and disease, the role of salivary gland mesenchyme is not well understood. In this study, we used the Col1a1-GFP mouse model to characterize the salivary gland mesenchyme in vitro and in vivo. The Col1a1-GFP transgene was exclusively expressed in the salivary gland mesenchyme. Ex vivo culture of mixed salivary gland cells in DMEM plus serum medium allowed long-term expansion of salivary gland epithelial and mesenchymal cells. The role of TGF- β 1 in salivary gland development and disease is complex. Therefore, we used this in vitro culture system to study the effects of TGF- β 1 on salivary gland cell differentiation. TGF- β 1 induced the expression of collagen, and inhibited the formation of acini-like structures in close proximity to mesenchymal cells, which adapted a fibroblastic phenotype. In contrast, TGF- β R1 inhibition increased acini genes and fibroblast growth factors (Fgf-7 and Fgf-10), decreased collagen and induced formation of larger, mature acini-like structures. Thus, inhibition of TGF- β signaling may be beneficial for salivary gland differentiation; however, due to differential effects of TGF- β 1 in salivary gland epithelial versus mesenchymal cells, selective inhibition is desirable. In conclusion, this mixed salivary gland cell culture system can be used to study epithelial-mesenchymal interactions and the effects of differentiating inducers and inhibitors. © 2013 Kajohnkiart Janebodin et al. 2014-08-29T09:17:50Z 2014-08-29T09:17:50Z 2013 Article 23146133 10.1155/2013/815895 23841093 http://www.scopus.com/inward/record.url?eid=2-s2.0-84880154193&partnerID=40&md5=c8a3a0c5e600b5fdff48177356bff7b6 http://cmuir.cmu.ac.th/handle/6653943832/1171 English |
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Despite a pivotal role in salivary gland development, homeostasis, and disease, the role of salivary gland mesenchyme is not well understood. In this study, we used the Col1a1-GFP mouse model to characterize the salivary gland mesenchyme in vitro and in vivo. The Col1a1-GFP transgene was exclusively expressed in the salivary gland mesenchyme. Ex vivo culture of mixed salivary gland cells in DMEM plus serum medium allowed long-term expansion of salivary gland epithelial and mesenchymal cells. The role of TGF- β 1 in salivary gland development and disease is complex. Therefore, we used this in vitro culture system to study the effects of TGF- β 1 on salivary gland cell differentiation. TGF- β 1 induced the expression of collagen, and inhibited the formation of acini-like structures in close proximity to mesenchymal cells, which adapted a fibroblastic phenotype. In contrast, TGF- β R1 inhibition increased acini genes and fibroblast growth factors (Fgf-7 and Fgf-10), decreased collagen and induced formation of larger, mature acini-like structures. Thus, inhibition of TGF- β signaling may be beneficial for salivary gland differentiation; however, due to differential effects of TGF- β 1 in salivary gland epithelial versus mesenchymal cells, selective inhibition is desirable. In conclusion, this mixed salivary gland cell culture system can be used to study epithelial-mesenchymal interactions and the effects of differentiating inducers and inhibitors. © 2013 Kajohnkiart Janebodin et al. |
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Article |
author |
Janebodin K. Buranaphatthana W. Ieronimakis N. Hays A.L. Reyes M. |
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Janebodin K. Buranaphatthana W. Ieronimakis N. Hays A.L. Reyes M. An in vitro culture system for long-term expansion of epithelial and mesenchymal salivary gland cells: Role of TGF- β 1 in salivary gland epithelial and mesenchymal differentiation |
author_facet |
Janebodin K. Buranaphatthana W. Ieronimakis N. Hays A.L. Reyes M. |
author_sort |
Janebodin K. |
title |
An in vitro culture system for long-term expansion of epithelial and mesenchymal salivary gland cells: Role of TGF- β 1 in salivary gland epithelial and mesenchymal differentiation |
title_short |
An in vitro culture system for long-term expansion of epithelial and mesenchymal salivary gland cells: Role of TGF- β 1 in salivary gland epithelial and mesenchymal differentiation |
title_full |
An in vitro culture system for long-term expansion of epithelial and mesenchymal salivary gland cells: Role of TGF- β 1 in salivary gland epithelial and mesenchymal differentiation |
title_fullStr |
An in vitro culture system for long-term expansion of epithelial and mesenchymal salivary gland cells: Role of TGF- β 1 in salivary gland epithelial and mesenchymal differentiation |
title_full_unstemmed |
An in vitro culture system for long-term expansion of epithelial and mesenchymal salivary gland cells: Role of TGF- β 1 in salivary gland epithelial and mesenchymal differentiation |
title_sort |
in vitro culture system for long-term expansion of epithelial and mesenchymal salivary gland cells: role of tgf- β 1 in salivary gland epithelial and mesenchymal differentiation |
publishDate |
2014 |
url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-84880154193&partnerID=40&md5=c8a3a0c5e600b5fdff48177356bff7b6 http://cmuir.cmu.ac.th/handle/6653943832/1171 |
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1681419620740759552 |