Crossing experiments of Anopheles minimus species C and putative species E

In the Anopheles minimus complex, 2 sibling species (A and C) are generally accepted. Recently, a 3rd species, provisionally designated An. minimus species E, has been described from the Ryukyu Archipelago, Japan, based on crossing experiments (A and E), DNA analysis, mitotic karyotypes, and some mo...

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Main Authors: Somboon P., Thongwat D., Choochote W., Walton C., Takagi M.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-16444362136&partnerID=40&md5=006d1c1777010427f1086c0c2c98da0c
http://www.ncbi.nlm.nih.gov/pubmed/15825754
http://cmuir.cmu.ac.th/handle/6653943832/1940
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spelling th-cmuir.6653943832-19402014-08-30T02:00:17Z Crossing experiments of Anopheles minimus species C and putative species E Somboon P. Thongwat D. Choochote W. Walton C. Takagi M. In the Anopheles minimus complex, 2 sibling species (A and C) are generally accepted. Recently, a 3rd species, provisionally designated An. minimus species E, has been described from the Ryukyu Archipelago, Japan, based on crossing experiments (A and E), DNA analysis, mitotic karyotypes, and some morphological characteristics. The present study reports the results of crossing experiments between species C and putative species E. Hybridization between the progeny of An. minimus species C from Thailand and putative species E from Japan revealed postzygotic genetic incompatibility. Although F1 hybrid progeny were obtained from both directions of crosses, the hybrid males from C female X E male crosses were completely sterile, with atrophied testes and accessory glands. In addition, the external terminalia of all of these males never completely rotated and the males failed to copulate by artificial mating. In E female X C male crosses, the hybrid males showed partially sterile testes in which most spermatozoa were abnormal (enlarged head) and inactive, and they had very little success in inseminating females. The salivary gland polytene chromosomes of F1 hybrid larvae from species C female X species E male showed a fixed heterozygous inversion on the 3L arm. Those F1 hybrids from species E female X species C male showed partial asynapsis on identified arms (2R and 3L) and a fixed heterozygous inversion on the 3R arm. When the F1 hybrid females from both directions of crosses were backcrossed with either C or E males, they produced male progeny with abnormal spermatozoa. Study of mating behavior in a 30 X 30 X 30-cm cage showed that the C males failed to mate with either C or E females, indicating that species C males cannot breed in confined spaces (lack stenogamy). Putative species E males had little success in inseminating species C females. This study provides strong evidence of genetic incompatibility between An. minimus species C and putative species E, supporting previous data that species E is a distinct species in the An. minimus complex. Copyright © 2005 by the American Mosquito Control Association, Inc. 2014-08-30T02:00:17Z 2014-08-30T02:00:17Z 2005 Article 8756971X 10.2987/8756-971X(2005)21[5:CEOAMS]2.0.CO;2 15825754 JAMAE http://www.scopus.com/inward/record.url?eid=2-s2.0-16444362136&partnerID=40&md5=006d1c1777010427f1086c0c2c98da0c http://www.ncbi.nlm.nih.gov/pubmed/15825754 http://cmuir.cmu.ac.th/handle/6653943832/1940 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description In the Anopheles minimus complex, 2 sibling species (A and C) are generally accepted. Recently, a 3rd species, provisionally designated An. minimus species E, has been described from the Ryukyu Archipelago, Japan, based on crossing experiments (A and E), DNA analysis, mitotic karyotypes, and some morphological characteristics. The present study reports the results of crossing experiments between species C and putative species E. Hybridization between the progeny of An. minimus species C from Thailand and putative species E from Japan revealed postzygotic genetic incompatibility. Although F1 hybrid progeny were obtained from both directions of crosses, the hybrid males from C female X E male crosses were completely sterile, with atrophied testes and accessory glands. In addition, the external terminalia of all of these males never completely rotated and the males failed to copulate by artificial mating. In E female X C male crosses, the hybrid males showed partially sterile testes in which most spermatozoa were abnormal (enlarged head) and inactive, and they had very little success in inseminating females. The salivary gland polytene chromosomes of F1 hybrid larvae from species C female X species E male showed a fixed heterozygous inversion on the 3L arm. Those F1 hybrids from species E female X species C male showed partial asynapsis on identified arms (2R and 3L) and a fixed heterozygous inversion on the 3R arm. When the F1 hybrid females from both directions of crosses were backcrossed with either C or E males, they produced male progeny with abnormal spermatozoa. Study of mating behavior in a 30 X 30 X 30-cm cage showed that the C males failed to mate with either C or E females, indicating that species C males cannot breed in confined spaces (lack stenogamy). Putative species E males had little success in inseminating species C females. This study provides strong evidence of genetic incompatibility between An. minimus species C and putative species E, supporting previous data that species E is a distinct species in the An. minimus complex. Copyright © 2005 by the American Mosquito Control Association, Inc.
format Article
author Somboon P.
Thongwat D.
Choochote W.
Walton C.
Takagi M.
spellingShingle Somboon P.
Thongwat D.
Choochote W.
Walton C.
Takagi M.
Crossing experiments of Anopheles minimus species C and putative species E
author_facet Somboon P.
Thongwat D.
Choochote W.
Walton C.
Takagi M.
author_sort Somboon P.
title Crossing experiments of Anopheles minimus species C and putative species E
title_short Crossing experiments of Anopheles minimus species C and putative species E
title_full Crossing experiments of Anopheles minimus species C and putative species E
title_fullStr Crossing experiments of Anopheles minimus species C and putative species E
title_full_unstemmed Crossing experiments of Anopheles minimus species C and putative species E
title_sort crossing experiments of anopheles minimus species c and putative species e
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-16444362136&partnerID=40&md5=006d1c1777010427f1086c0c2c98da0c
http://www.ncbi.nlm.nih.gov/pubmed/15825754
http://cmuir.cmu.ac.th/handle/6653943832/1940
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