Use of different PCR primers and gastric biopsy tissue from CLO test for the detection of Helicobacter pylori

• Main Authors: Linpisarn S., Koosirirat C., Prommuangyong K., Suwan W., Lertprasertsuke N., Phornphutkul K.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-17744382227&partnerID=40&md5=0486ad49d87f498fbbf09ca2432ac258; http://www.ncbi.nlm.nih.gov/pubmed/15906656; http://cmuir.cmu.ac.th/handle/6653943832/1972
• Institution: Chiang Mai University

Four different DNA loci were assessed for the detection of H. pylori by PCR on gastric biopsy specimens. PCR, with a primer specific 860 bp DNA fragment, was the most sensitive, with a detection limit of 0.02 pg H. pylori DNA, corresponding to approximately 10 organisms. Nested-PCR of the 860-bp DNA fragment was 10-fold more sensitive than single-step PCR. The sensitivity and specificity of the four PCR methods, in comparison to the results obtained from histology and the urease test, are as follows: 80.7% and 76% for the hpaA gene; 100% and 76% for the 16S rRNA gene; 84.6% and 80.0% for the 860-bp DNA fragment; 61.5% and 84.0% for the ureC (glmM) gene, respectively. The sensitivity of nested-PCR for the 860-bp DNA fragment was 100%. This nested-PCR gave positive results for eight specimens which were negative by conventional methods. PCR can be performed on gastric biopsy specimens obtained from the CLO test.