In vitro excystation of Haplorchis taichui (Trematoda: Heterophyidae)

The effects of trypsin, bile extract, temperature and acid-based condition for the in vitro excystation of Haplorchis taichui metacercariae were studied. At 37°C, approximately half the number of metacercariae excysted when exposed to 1% trypsin for 15 minutes with no more excystation found beyond t...

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Main Authors: Sukontason K., Boonsriwong N., Chaithong U., Sukontason K.L., Piangjai S.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-0035554424&partnerID=40&md5=cdf987b3b9b4e4546b6a0d6e1ca07a5d
http://www.ncbi.nlm.nih.gov/pubmed/12041599
http://cmuir.cmu.ac.th/handle/6653943832/1976
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-19762014-08-30T02:00:20Z In vitro excystation of Haplorchis taichui (Trematoda: Heterophyidae) Sukontason K. Boonsriwong N. Chaithong U. Sukontason K.L. Piangjai S. The effects of trypsin, bile extract, temperature and acid-based condition for the in vitro excystation of Haplorchis taichui metacercariae were studied. At 37°C, approximately half the number of metacercariae excysted when exposed to 1% trypsin for 15 minutes with no more excystation found beyond this time. Increasing trypsin concentration seemed to reduce the excystation rate while bile extract was, however, unlikely to be an absolute requirement. A temperature of 37°-41°C yielded a similar excystation result in combination with 1% trypsin; however, less excystation occurred at a lower temperature of 35°C. The acid-based environment of pH 8 gave the best excystation result in association with 1% trypsin at a temperature of 39°C. Higher and lower basicity produced a smaller excystation rate. An environmental condition of 1% trypsin at pH 8 and a temperature of between 37°-41°C was recommended for the in vitro excystation of H. taichui metacercariae. The relatively broad temperature and pH range condition for the excystation of H. taichui corresponded with various definitive hosts that were infected naturally by this fluke. 2014-08-30T02:00:20Z 2014-08-30T02:00:20Z 2001 Article 01251562 12041599 http://www.scopus.com/inward/record.url?eid=2-s2.0-0035554424&partnerID=40&md5=cdf987b3b9b4e4546b6a0d6e1ca07a5d http://www.ncbi.nlm.nih.gov/pubmed/12041599 http://cmuir.cmu.ac.th/handle/6653943832/1976 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description The effects of trypsin, bile extract, temperature and acid-based condition for the in vitro excystation of Haplorchis taichui metacercariae were studied. At 37°C, approximately half the number of metacercariae excysted when exposed to 1% trypsin for 15 minutes with no more excystation found beyond this time. Increasing trypsin concentration seemed to reduce the excystation rate while bile extract was, however, unlikely to be an absolute requirement. A temperature of 37°-41°C yielded a similar excystation result in combination with 1% trypsin; however, less excystation occurred at a lower temperature of 35°C. The acid-based environment of pH 8 gave the best excystation result in association with 1% trypsin at a temperature of 39°C. Higher and lower basicity produced a smaller excystation rate. An environmental condition of 1% trypsin at pH 8 and a temperature of between 37°-41°C was recommended for the in vitro excystation of H. taichui metacercariae. The relatively broad temperature and pH range condition for the excystation of H. taichui corresponded with various definitive hosts that were infected naturally by this fluke.
format Article
author Sukontason K.
Boonsriwong N.
Chaithong U.
Sukontason K.L.
Piangjai S.
spellingShingle Sukontason K.
Boonsriwong N.
Chaithong U.
Sukontason K.L.
Piangjai S.
In vitro excystation of Haplorchis taichui (Trematoda: Heterophyidae)
author_facet Sukontason K.
Boonsriwong N.
Chaithong U.
Sukontason K.L.
Piangjai S.
author_sort Sukontason K.
title In vitro excystation of Haplorchis taichui (Trematoda: Heterophyidae)
title_short In vitro excystation of Haplorchis taichui (Trematoda: Heterophyidae)
title_full In vitro excystation of Haplorchis taichui (Trematoda: Heterophyidae)
title_fullStr In vitro excystation of Haplorchis taichui (Trematoda: Heterophyidae)
title_full_unstemmed In vitro excystation of Haplorchis taichui (Trematoda: Heterophyidae)
title_sort in vitro excystation of haplorchis taichui (trematoda: heterophyidae)
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-0035554424&partnerID=40&md5=cdf987b3b9b4e4546b6a0d6e1ca07a5d
http://www.ncbi.nlm.nih.gov/pubmed/12041599
http://cmuir.cmu.ac.th/handle/6653943832/1976
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