Expression of human lactoferrin in Bacteroides uniformis and its effect on azoxymethane-induced aberrant crypt focus formation in the rat colon

• Main Authors: Chewonarin T., Kuwahara T., Arimochi H., Kataoka K., Nakayama H., Yu D.-Y., Tsuda H., Vinitketkumnuen U., Ohnishi Y.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-0035700157&partnerID=40&md5=79db87d898f5c6dbf5d25058acca3210; http://cmuir.cmu.ac.th/handle/6653943832/2008
• Institution: Chiang Mai University

To express a human lactoferrin (hLF) gene in Bacteroides uniformis, a member of the anaerobic microflora in the colon, we constructed a recombinant plasmid, pVLFK, by subcloning hLF cDNA to an Escherichia coli-Bacteroides shuttle vector, pVAL-1. The plasmid pVLFK was transferred to B. uniformis stra...