Intraspecific hybridization of Anopheles minimus (Diptera: Culicidae) species A and C in Thailand.

Hybridization tests of laboratory-raised, isolines of Anopheles minimus, species A and C were conducted by induced copulation. The three isolines were established based on three morphological variants of wild-caught, fully engorged females and two distinct types of metaphase chromosomes. They were A...

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Main Authors: Choochote W., Rongsriyam Y., Leemingsawat S., Jitpakdi A., Komalamisra N., Surathin K., Somboon P., Chen B., Wongkamchai S., Jariyapan N., Tippawangkosol P., Pitasawat B., Riyong D.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-18144451927&partnerID=40&md5=5f76b86e0958bb074caa90abd8298d9c
http://cmuir.cmu.ac.th/handle/6653943832/2181
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-21812014-08-30T02:00:34Z Intraspecific hybridization of Anopheles minimus (Diptera: Culicidae) species A and C in Thailand. Choochote W. Rongsriyam Y. Leemingsawat S. Jitpakdi A. Komalamisra N. Surathin K. Somboon P. Chen B. Wongkamchai S. Jariyapan N. Tippawangkosol P. Pitasawat B. Riyong D. Hybridization tests of laboratory-raised, isolines of Anopheles minimus, species A and C were conducted by induced copulation. The three isolines were established based on three morphological variants of wild-caught, fully engorged females and two distinct types of metaphase chromosomes. They were An. minimus species A: V form (X1,Y1), M form (X2,Y1); species C: P form (X3,Y2). The results of reciprocal and back crosses indicated that the two morphologically variant forms of species A were genetically compatible, providing viable progeny and completely synaptic salivary gland polytene chromosomes, whereas they were genetically incompatible with species C and/or the P form. Hybrid progeny was only obtained from both forms of species A females x species C males, but asynaptic salivary gland polytene chromosomes on 3L and partial development of ovarian follicles in females were seen. Back crosses of F1 hybrid males with parental species A females provided viable progeny, while back crosses of F1 hybrid females with parental species C males provided progeny of low viability and adult males with abnormal spermatozoa, suggesting the partial reproductive isolation of An. minimus species A and C. 2014-08-30T02:00:34Z 2014-08-30T02:00:34Z 2002 Article 01251562 12971469 http://www.scopus.com/inward/record.url?eid=2-s2.0-18144451927&partnerID=40&md5=5f76b86e0958bb074caa90abd8298d9c http://cmuir.cmu.ac.th/handle/6653943832/2181 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Hybridization tests of laboratory-raised, isolines of Anopheles minimus, species A and C were conducted by induced copulation. The three isolines were established based on three morphological variants of wild-caught, fully engorged females and two distinct types of metaphase chromosomes. They were An. minimus species A: V form (X1,Y1), M form (X2,Y1); species C: P form (X3,Y2). The results of reciprocal and back crosses indicated that the two morphologically variant forms of species A were genetically compatible, providing viable progeny and completely synaptic salivary gland polytene chromosomes, whereas they were genetically incompatible with species C and/or the P form. Hybrid progeny was only obtained from both forms of species A females x species C males, but asynaptic salivary gland polytene chromosomes on 3L and partial development of ovarian follicles in females were seen. Back crosses of F1 hybrid males with parental species A females provided viable progeny, while back crosses of F1 hybrid females with parental species C males provided progeny of low viability and adult males with abnormal spermatozoa, suggesting the partial reproductive isolation of An. minimus species A and C.
format Article
author Choochote W.
Rongsriyam Y.
Leemingsawat S.
Jitpakdi A.
Komalamisra N.
Surathin K.
Somboon P.
Chen B.
Wongkamchai S.
Jariyapan N.
Tippawangkosol P.
Pitasawat B.
Riyong D.
spellingShingle Choochote W.
Rongsriyam Y.
Leemingsawat S.
Jitpakdi A.
Komalamisra N.
Surathin K.
Somboon P.
Chen B.
Wongkamchai S.
Jariyapan N.
Tippawangkosol P.
Pitasawat B.
Riyong D.
Intraspecific hybridization of Anopheles minimus (Diptera: Culicidae) species A and C in Thailand.
author_facet Choochote W.
Rongsriyam Y.
Leemingsawat S.
Jitpakdi A.
Komalamisra N.
Surathin K.
Somboon P.
Chen B.
Wongkamchai S.
Jariyapan N.
Tippawangkosol P.
Pitasawat B.
Riyong D.
author_sort Choochote W.
title Intraspecific hybridization of Anopheles minimus (Diptera: Culicidae) species A and C in Thailand.
title_short Intraspecific hybridization of Anopheles minimus (Diptera: Culicidae) species A and C in Thailand.
title_full Intraspecific hybridization of Anopheles minimus (Diptera: Culicidae) species A and C in Thailand.
title_fullStr Intraspecific hybridization of Anopheles minimus (Diptera: Culicidae) species A and C in Thailand.
title_full_unstemmed Intraspecific hybridization of Anopheles minimus (Diptera: Culicidae) species A and C in Thailand.
title_sort intraspecific hybridization of anopheles minimus (diptera: culicidae) species a and c in thailand.
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-18144451927&partnerID=40&md5=5f76b86e0958bb074caa90abd8298d9c
http://cmuir.cmu.ac.th/handle/6653943832/2181
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