Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis
In 2004, an epidemiological survey of human rotavirus infection in Chiang Mai, Thailand detected two uncommon human rotavirus strains (CMH120/04 and CMH134/04) bearing AU-1-like G3P[9] genotypes in 1 year old children hospitalized with acute gastroenteritis. The CMH120/04 and CMH134/04 rotavirus str...
Saved in:
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
2014
|
Online Access: | http://www.scopus.com/inward/record.url?eid=2-s2.0-34248167778&partnerID=40&md5=c3d3a658e7822aecee28ab4a19df4e19 http://cmuir.cmu.ac.th/handle/6653943832/2206 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Chiang Mai University |
Language: | English |
id |
th-cmuir.6653943832-2206 |
---|---|
record_format |
dspace |
spelling |
th-cmuir.6653943832-22062014-08-30T02:00:35Z Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis Khamrin P. Maneekarn N. Peerakome S. Tonusin S. Tung G.P. Okitsu S. Ushijima H. In 2004, an epidemiological survey of human rotavirus infection in Chiang Mai, Thailand detected two uncommon human rotavirus strains (CMH120/04 and CMH134/04) bearing AU-1-like G3P[9] genotypes in 1 year old children hospitalized with acute gastroenteritis. The CMH120/04 and CMH134/04 rotavirus strains were characterized by molecular analyses of their VP6, VP7, VP8*, and NSP4 gene segments as well as the determination of RNA patterns by polyacrylamide gel electrophoresis (PAGE). Analysis of the VP8* gene revealed a high level of amino acid sequence identities with those of P[9] rotavirus reference strains, ranging from 94.9% to 98.3%. The highest identities were shared with the human rotavirus AU-1 strain at 97.8% and 98.3% for CMH120/04 and CMH134/04 strains, respectively. Analysis of the VP7 gene sequence revealed the highest identities with G3 human rotavirus strain KC814 at 96.6% and 96.2% for CMH120/04 and CMH134/ 04 strains, respectively. Based on the analyses of VP7 and VP8* genes, CMH120/04 and CMH134/04 belonged to G3P[9] genotypes. In addition, analyses of VP6 and NSP4 sequences revealed a VP6 subgroup (SG) I, with NSP4 genetic group C specificities. Moreover, both strains displayed a long RNA electrophoretic pattern. The finding of uncommon G3P[9] rotaviruses in pediatric patients provided additional evidence of the genetic/antigenic diversities of human group A rotaviruses in the Chiang Mai area of Thailand. © 2007 Wiley-Liss, Inc. 2014-08-30T02:00:35Z 2014-08-30T02:00:35Z 2007 Article 01466615 10.1002/jmv.20840 17457907 JMVID http://www.scopus.com/inward/record.url?eid=2-s2.0-34248167778&partnerID=40&md5=c3d3a658e7822aecee28ab4a19df4e19 http://cmuir.cmu.ac.th/handle/6653943832/2206 English |
institution |
Chiang Mai University |
building |
Chiang Mai University Library |
country |
Thailand |
collection |
CMU Intellectual Repository |
language |
English |
description |
In 2004, an epidemiological survey of human rotavirus infection in Chiang Mai, Thailand detected two uncommon human rotavirus strains (CMH120/04 and CMH134/04) bearing AU-1-like G3P[9] genotypes in 1 year old children hospitalized with acute gastroenteritis. The CMH120/04 and CMH134/04 rotavirus strains were characterized by molecular analyses of their VP6, VP7, VP8*, and NSP4 gene segments as well as the determination of RNA patterns by polyacrylamide gel electrophoresis (PAGE). Analysis of the VP8* gene revealed a high level of amino acid sequence identities with those of P[9] rotavirus reference strains, ranging from 94.9% to 98.3%. The highest identities were shared with the human rotavirus AU-1 strain at 97.8% and 98.3% for CMH120/04 and CMH134/04 strains, respectively. Analysis of the VP7 gene sequence revealed the highest identities with G3 human rotavirus strain KC814 at 96.6% and 96.2% for CMH120/04 and CMH134/ 04 strains, respectively. Based on the analyses of VP7 and VP8* genes, CMH120/04 and CMH134/04 belonged to G3P[9] genotypes. In addition, analyses of VP6 and NSP4 sequences revealed a VP6 subgroup (SG) I, with NSP4 genetic group C specificities. Moreover, both strains displayed a long RNA electrophoretic pattern. The finding of uncommon G3P[9] rotaviruses in pediatric patients provided additional evidence of the genetic/antigenic diversities of human group A rotaviruses in the Chiang Mai area of Thailand. © 2007 Wiley-Liss, Inc. |
format |
Article |
author |
Khamrin P. Maneekarn N. Peerakome S. Tonusin S. Tung G.P. Okitsu S. Ushijima H. |
spellingShingle |
Khamrin P. Maneekarn N. Peerakome S. Tonusin S. Tung G.P. Okitsu S. Ushijima H. Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis |
author_facet |
Khamrin P. Maneekarn N. Peerakome S. Tonusin S. Tung G.P. Okitsu S. Ushijima H. |
author_sort |
Khamrin P. |
title |
Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis |
title_short |
Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis |
title_full |
Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis |
title_fullStr |
Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis |
title_full_unstemmed |
Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis |
title_sort |
molecular characterization of rare g3p[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis |
publishDate |
2014 |
url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-34248167778&partnerID=40&md5=c3d3a658e7822aecee28ab4a19df4e19 http://cmuir.cmu.ac.th/handle/6653943832/2206 |
_version_ |
1681419814988414976 |