Genetic diversity and molecular identification of mosquito species in the Anopheles maculatus group using the ITS2 region of rDNA

The species diversity and genetic structure of mosquitoes belonging to the Anopheles maculatus group in Southeast Asia were investigated using the internal transcribed spacer 2 (ITS2) of ribosomal DNA (rDNA). A molecular phylogeny indicates the presence of at least one hitherto unrecognised species....

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Main Authors: Walton C., Somboon P., O'Loughlin S.M., Zhang S., Harbach R.E., Linton Y.-M., Chen B., Nolan K., Duong S., Fong M.-Y., Vythilingum I., Mohammed Z.D., Trung H.D., Butlin R.K.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-33845264599&partnerID=40&md5=382ef84186e1a2f4d5a01ebb8178a140
http://cmuir.cmu.ac.th/handle/6653943832/2288
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spelling th-cmuir.6653943832-22882014-08-30T02:00:41Z Genetic diversity and molecular identification of mosquito species in the Anopheles maculatus group using the ITS2 region of rDNA Walton C. Somboon P. O'Loughlin S.M. Zhang S. Harbach R.E. Linton Y.-M. Chen B. Nolan K. Duong S. Fong M.-Y. Vythilingum I. Mohammed Z.D. Trung H.D. Butlin R.K. The species diversity and genetic structure of mosquitoes belonging to the Anopheles maculatus group in Southeast Asia were investigated using the internal transcribed spacer 2 (ITS2) of ribosomal DNA (rDNA). A molecular phylogeny indicates the presence of at least one hitherto unrecognised species. Mosquitoes of chromosomal form K from eastern Thailand have a unique ITS2 sequence that is 3.7% divergent from the next most closely related taxon (An. sawadwongporni) in the group. In the context of negligible intraspecific variation at ITS2, this suggests that chromosomal form K is most probably a distinct species. Although An. maculatus sensu stricto from northern Thailand and southern Thailand/peninsular Malaysia differ from each other in chromosomal banding pattern and vectorial capacity, no intraspecific variation was observed in the ITS2 sequences of this species over this entire geographic area despite an extensive survey. A PCR-based identification method was developed to distinguish five species of the group (An. maculatus, An. dravidicus, An. pseudowillmori, An. sawadwongporni and chromosomal form K) to assist field-based studies in northwestern Thailand. Sequences from 187 mosquitoes (mostly An. maculatus and An. sawadwongporni) revealed no intraspecific variation in specimens from Thailand, Cambodia, mainland China, Malaysia, Taiwan and Vietnam, suggesting that this identification method will be widely applicable in Southeast Asia. The lack of detectable genetic structure also suggests that populations of these species are either connected by gene flow and/or share a recent common history. © 2006 Elsevier B.V. All rights reserved. 2014-08-30T02:00:41Z 2014-08-30T02:00:41Z 2007 Article 15671348 10.1016/j.meegid.2006.05.001 16782411 IGENC http://www.scopus.com/inward/record.url?eid=2-s2.0-33845264599&partnerID=40&md5=382ef84186e1a2f4d5a01ebb8178a140 http://cmuir.cmu.ac.th/handle/6653943832/2288 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description The species diversity and genetic structure of mosquitoes belonging to the Anopheles maculatus group in Southeast Asia were investigated using the internal transcribed spacer 2 (ITS2) of ribosomal DNA (rDNA). A molecular phylogeny indicates the presence of at least one hitherto unrecognised species. Mosquitoes of chromosomal form K from eastern Thailand have a unique ITS2 sequence that is 3.7% divergent from the next most closely related taxon (An. sawadwongporni) in the group. In the context of negligible intraspecific variation at ITS2, this suggests that chromosomal form K is most probably a distinct species. Although An. maculatus sensu stricto from northern Thailand and southern Thailand/peninsular Malaysia differ from each other in chromosomal banding pattern and vectorial capacity, no intraspecific variation was observed in the ITS2 sequences of this species over this entire geographic area despite an extensive survey. A PCR-based identification method was developed to distinguish five species of the group (An. maculatus, An. dravidicus, An. pseudowillmori, An. sawadwongporni and chromosomal form K) to assist field-based studies in northwestern Thailand. Sequences from 187 mosquitoes (mostly An. maculatus and An. sawadwongporni) revealed no intraspecific variation in specimens from Thailand, Cambodia, mainland China, Malaysia, Taiwan and Vietnam, suggesting that this identification method will be widely applicable in Southeast Asia. The lack of detectable genetic structure also suggests that populations of these species are either connected by gene flow and/or share a recent common history. © 2006 Elsevier B.V. All rights reserved.
format Article
author Walton C.
Somboon P.
O'Loughlin S.M.
Zhang S.
Harbach R.E.
Linton Y.-M.
Chen B.
Nolan K.
Duong S.
Fong M.-Y.
Vythilingum I.
Mohammed Z.D.
Trung H.D.
Butlin R.K.
spellingShingle Walton C.
Somboon P.
O'Loughlin S.M.
Zhang S.
Harbach R.E.
Linton Y.-M.
Chen B.
Nolan K.
Duong S.
Fong M.-Y.
Vythilingum I.
Mohammed Z.D.
Trung H.D.
Butlin R.K.
Genetic diversity and molecular identification of mosquito species in the Anopheles maculatus group using the ITS2 region of rDNA
author_facet Walton C.
Somboon P.
O'Loughlin S.M.
Zhang S.
Harbach R.E.
Linton Y.-M.
Chen B.
Nolan K.
Duong S.
Fong M.-Y.
Vythilingum I.
Mohammed Z.D.
Trung H.D.
Butlin R.K.
author_sort Walton C.
title Genetic diversity and molecular identification of mosquito species in the Anopheles maculatus group using the ITS2 region of rDNA
title_short Genetic diversity and molecular identification of mosquito species in the Anopheles maculatus group using the ITS2 region of rDNA
title_full Genetic diversity and molecular identification of mosquito species in the Anopheles maculatus group using the ITS2 region of rDNA
title_fullStr Genetic diversity and molecular identification of mosquito species in the Anopheles maculatus group using the ITS2 region of rDNA
title_full_unstemmed Genetic diversity and molecular identification of mosquito species in the Anopheles maculatus group using the ITS2 region of rDNA
title_sort genetic diversity and molecular identification of mosquito species in the anopheles maculatus group using the its2 region of rdna
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-33845264599&partnerID=40&md5=382ef84186e1a2f4d5a01ebb8178a140
http://cmuir.cmu.ac.th/handle/6653943832/2288
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