Morphology of immature stages of Hemipyrellia ligurriens (Wiedemann) (Diptera: Calliphoridae) for use in forensic entomology applications

In forensic investigations, all immature stages of flies (egg, larvae, and puparium) can serve as entomological evidence at death scenes. These insects are primarily used to estimate the post mortem interval (PMI), but can also be involved in the analysis of toxic substances, determining manner of d...

Full description

Saved in:
Bibliographic Details
Main Authors: Sukontason K.L., Sribanditmongkol P., Chaiwong T., Vogtsberger R.C., Piangjai S., Sukontason K.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-48349087124&partnerID=40&md5=56c3c8e87e7d98561a09fd439b34f254
http://www.ncbi.nlm.nih.gov/pubmed/18568445
http://cmuir.cmu.ac.th/handle/6653943832/2384
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
Language: English
id th-cmuir.6653943832-2384
record_format dspace
spelling th-cmuir.6653943832-23842014-08-30T02:00:47Z Morphology of immature stages of Hemipyrellia ligurriens (Wiedemann) (Diptera: Calliphoridae) for use in forensic entomology applications Sukontason K.L. Sribanditmongkol P. Chaiwong T. Vogtsberger R.C. Piangjai S. Sukontason K. In forensic investigations, all immature stages of flies (egg, larvae, and puparium) can serve as entomological evidence at death scenes. These insects are primarily used to estimate the post mortem interval (PMI), but can also be involved in the analysis of toxic substances, determining manner of death, and in indicating relocation of a corpse in homicide cases. In this study, we present the morphology of the egg, larvae, and puparium of Hemipyrellia ligurriens, a blow fly species of forensic importance in Thailand. Examination was conducted using scanning electron microscopy (SEM). The egg stage was found to display a relatively wide plastron region (or median hatch line area) that spans almost the entire length of the egg. The median hatch line is oriented in an upright position. External chorionic sculpture of the egg is present in a hexagonal pattern whose reticular boundaries are slightly elevated. In the larval stages, the most prominent morphological changes were detected upon comparison of the first to the second instar; whereas, the differences between second and third instar larvae were less obvious outside of the increase in number of posterior spiracular slits. Most of the major differences involve body size and structure of the anterior and posterior spiracles. Each anterior spiracle in both the second and third instars projects five to seven papillae apically. Each posterior spiracular disc of a third instar exhibits a complete peritreme, three spiracular slits, and a prominent button that is ventromedially located. The puparium is coarctate and features a clustered bubble membrane comprised of ≈57 mammillate structures positioned dorsolaterally on each side of the first abdominal segment in young puparia. This feature is replaced by short, tubular respiratory horns in aged puparia. This study provides more detailed exposure of important morphological features that can be used for accurate identification of immature stages of H. ligurriens. Information presented can aid in forensic investigations involving this fly species. © 2008 Springer-Verlag. 2014-08-30T02:00:47Z 2014-08-30T02:00:47Z 2008 Article 09320113 10.1007/s00436-008-1072-7 18568445 PARRE http://www.scopus.com/inward/record.url?eid=2-s2.0-48349087124&partnerID=40&md5=56c3c8e87e7d98561a09fd439b34f254 http://www.ncbi.nlm.nih.gov/pubmed/18568445 http://cmuir.cmu.ac.th/handle/6653943832/2384 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description In forensic investigations, all immature stages of flies (egg, larvae, and puparium) can serve as entomological evidence at death scenes. These insects are primarily used to estimate the post mortem interval (PMI), but can also be involved in the analysis of toxic substances, determining manner of death, and in indicating relocation of a corpse in homicide cases. In this study, we present the morphology of the egg, larvae, and puparium of Hemipyrellia ligurriens, a blow fly species of forensic importance in Thailand. Examination was conducted using scanning electron microscopy (SEM). The egg stage was found to display a relatively wide plastron region (or median hatch line area) that spans almost the entire length of the egg. The median hatch line is oriented in an upright position. External chorionic sculpture of the egg is present in a hexagonal pattern whose reticular boundaries are slightly elevated. In the larval stages, the most prominent morphological changes were detected upon comparison of the first to the second instar; whereas, the differences between second and third instar larvae were less obvious outside of the increase in number of posterior spiracular slits. Most of the major differences involve body size and structure of the anterior and posterior spiracles. Each anterior spiracle in both the second and third instars projects five to seven papillae apically. Each posterior spiracular disc of a third instar exhibits a complete peritreme, three spiracular slits, and a prominent button that is ventromedially located. The puparium is coarctate and features a clustered bubble membrane comprised of ≈57 mammillate structures positioned dorsolaterally on each side of the first abdominal segment in young puparia. This feature is replaced by short, tubular respiratory horns in aged puparia. This study provides more detailed exposure of important morphological features that can be used for accurate identification of immature stages of H. ligurriens. Information presented can aid in forensic investigations involving this fly species. © 2008 Springer-Verlag.
format Article
author Sukontason K.L.
Sribanditmongkol P.
Chaiwong T.
Vogtsberger R.C.
Piangjai S.
Sukontason K.
spellingShingle Sukontason K.L.
Sribanditmongkol P.
Chaiwong T.
Vogtsberger R.C.
Piangjai S.
Sukontason K.
Morphology of immature stages of Hemipyrellia ligurriens (Wiedemann) (Diptera: Calliphoridae) for use in forensic entomology applications
author_facet Sukontason K.L.
Sribanditmongkol P.
Chaiwong T.
Vogtsberger R.C.
Piangjai S.
Sukontason K.
author_sort Sukontason K.L.
title Morphology of immature stages of Hemipyrellia ligurriens (Wiedemann) (Diptera: Calliphoridae) for use in forensic entomology applications
title_short Morphology of immature stages of Hemipyrellia ligurriens (Wiedemann) (Diptera: Calliphoridae) for use in forensic entomology applications
title_full Morphology of immature stages of Hemipyrellia ligurriens (Wiedemann) (Diptera: Calliphoridae) for use in forensic entomology applications
title_fullStr Morphology of immature stages of Hemipyrellia ligurriens (Wiedemann) (Diptera: Calliphoridae) for use in forensic entomology applications
title_full_unstemmed Morphology of immature stages of Hemipyrellia ligurriens (Wiedemann) (Diptera: Calliphoridae) for use in forensic entomology applications
title_sort morphology of immature stages of hemipyrellia ligurriens (wiedemann) (diptera: calliphoridae) for use in forensic entomology applications
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-48349087124&partnerID=40&md5=56c3c8e87e7d98561a09fd439b34f254
http://www.ncbi.nlm.nih.gov/pubmed/18568445
http://cmuir.cmu.ac.th/handle/6653943832/2384
_version_ 1681419848545992704