Involvement of phospholipase D in regulating expression of anti-microbial peptide human β-defensin-2

Human β-defensin expression correlates with differentiation in oral epithelium, and calcium ion, an important regulator of epithelial differentiation, plays a critical role in regulation of human β-defensin-2 (hBD-2) mRNA expression. Phospholipase D (PLD) also regulates epithelial differentiation. T...

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Main Authors: Krisanaprakornkit S., Chotjumlong P., Kongtawelert P., Reutrakul V.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-37549064726&partnerID=40&md5=d4053f1691c1ea02838ce91d5be4f13f
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spelling th-cmuir.6653943832-25012014-08-30T02:00:55Z Involvement of phospholipase D in regulating expression of anti-microbial peptide human β-defensin-2 Krisanaprakornkit S. Chotjumlong P. Kongtawelert P. Reutrakul V. Human β-defensin expression correlates with differentiation in oral epithelium, and calcium ion, an important regulator of epithelial differentiation, plays a critical role in regulation of human β-defensin-2 (hBD-2) mRNA expression. Phospholipase D (PLD) also regulates epithelial differentiation. Therefore, we examined the role of PLD in hBD-2 up-regulation by cell wall extract of Fusobacterium nucleatum and phorbol 12-myristate 13-acetate (PMA), two known hBD-2 activators. We found that hBD-2 mRNA up-regulation in human gingival epithelial cells (HGECs) by these two activators was mediated by PLD activation and blocked by ethanol and 1-butanol, PLD inhibitors. PLD activity was induced by stimulation with these two activators, and phosphatidic acid (PA), a product generated from the PLD enzymatic activity, was detected in stimulated HGECs. Dioctanoyl PA commonly used for PA induced hBD-2 mRNA expression. mRNAs for PLD1α and β splice variants as well as PLD1 protein were constitutively expressed, whereas mRNA and protein for PLD2 were expressed at much lower levels than those for PLD1. Moreover, pre-treatment with (±)-propanolol, an inhibitor of phosphatidic acid phosphohydrolases that are the downstream signaling molecules in the PLD pathway, significantly blocked hBD-2 mRNA induction by PMA in a dose-dependent manner. In conclusion, these findings indicate the involvement of PLD activation in hBD-2 up-regulation in HGECs, which correlates with the state of epithelial differentiation. © The Japanese Society for Immunology. 2007. All rights reserved. 2014-08-30T02:00:55Z 2014-08-30T02:00:55Z 2008 Article 09538178 10.1093/intimm/dxm115 17986621 INIME http://www.scopus.com/inward/record.url?eid=2-s2.0-37549064726&partnerID=40&md5=d4053f1691c1ea02838ce91d5be4f13f http://cmuir.cmu.ac.th/handle/6653943832/2501 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
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language English
description Human β-defensin expression correlates with differentiation in oral epithelium, and calcium ion, an important regulator of epithelial differentiation, plays a critical role in regulation of human β-defensin-2 (hBD-2) mRNA expression. Phospholipase D (PLD) also regulates epithelial differentiation. Therefore, we examined the role of PLD in hBD-2 up-regulation by cell wall extract of Fusobacterium nucleatum and phorbol 12-myristate 13-acetate (PMA), two known hBD-2 activators. We found that hBD-2 mRNA up-regulation in human gingival epithelial cells (HGECs) by these two activators was mediated by PLD activation and blocked by ethanol and 1-butanol, PLD inhibitors. PLD activity was induced by stimulation with these two activators, and phosphatidic acid (PA), a product generated from the PLD enzymatic activity, was detected in stimulated HGECs. Dioctanoyl PA commonly used for PA induced hBD-2 mRNA expression. mRNAs for PLD1α and β splice variants as well as PLD1 protein were constitutively expressed, whereas mRNA and protein for PLD2 were expressed at much lower levels than those for PLD1. Moreover, pre-treatment with (±)-propanolol, an inhibitor of phosphatidic acid phosphohydrolases that are the downstream signaling molecules in the PLD pathway, significantly blocked hBD-2 mRNA induction by PMA in a dose-dependent manner. In conclusion, these findings indicate the involvement of PLD activation in hBD-2 up-regulation in HGECs, which correlates with the state of epithelial differentiation. © The Japanese Society for Immunology. 2007. All rights reserved.
format Article
author Krisanaprakornkit S.
Chotjumlong P.
Kongtawelert P.
Reutrakul V.
spellingShingle Krisanaprakornkit S.
Chotjumlong P.
Kongtawelert P.
Reutrakul V.
Involvement of phospholipase D in regulating expression of anti-microbial peptide human β-defensin-2
author_facet Krisanaprakornkit S.
Chotjumlong P.
Kongtawelert P.
Reutrakul V.
author_sort Krisanaprakornkit S.
title Involvement of phospholipase D in regulating expression of anti-microbial peptide human β-defensin-2
title_short Involvement of phospholipase D in regulating expression of anti-microbial peptide human β-defensin-2
title_full Involvement of phospholipase D in regulating expression of anti-microbial peptide human β-defensin-2
title_fullStr Involvement of phospholipase D in regulating expression of anti-microbial peptide human β-defensin-2
title_full_unstemmed Involvement of phospholipase D in regulating expression of anti-microbial peptide human β-defensin-2
title_sort involvement of phospholipase d in regulating expression of anti-microbial peptide human β-defensin-2
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-37549064726&partnerID=40&md5=d4053f1691c1ea02838ce91d5be4f13f
http://cmuir.cmu.ac.th/handle/6653943832/2501
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