Influence of pr-M cleavage on the heterogeneity of extracellular dengue virus particles
During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular pa...
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| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2014
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| Online Access: | http://www.scopus.com/inward/record.url?eid=2-s2.0-77954972691&partnerID=40&md5=fa4517b9d33254b25831e268eaf768a6 http://www.ncbi.nlm.nih.gov/pubmed/20519400 http://cmuir.cmu.ac.th/handle/6653943832/2555 |
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| Institution: | Chiang Mai University |
| Language: | English |
| Summary: | During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partially mature particles, as the major prM-containing particles in a dengue serotype 2 virus. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage. Copyright © 2010, American Society for Microbiology. All Rights Reserved. |
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