Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos

Objective: To study the effect of embryo density and microdrop volume on mouse two-cell embryo development. Design: Experimental study. Setting: Assisted conception laboratory. Animal(s): Two-cell mouse embryos (n = 1511). Intervention(s): One, five, 10, or 15 embryos were cultured in 10-μL drops of...

Full description

Saved in:
Bibliographic Details
Main Authors: Vutyavanich T., Saeng-Anan U., Sirisukkasem S., Piromlertamorn W.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-79952448537&partnerID=40&md5=f919357c08b79d77d1302efa2ecf5d19
http://www.ncbi.nlm.nih.gov/pubmed/20561617
http://cmuir.cmu.ac.th/handle/6653943832/2684
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
Language: English
id th-cmuir.6653943832-2684
record_format dspace
spelling th-cmuir.6653943832-26842014-08-30T02:25:16Z Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos Vutyavanich T. Saeng-Anan U. Sirisukkasem S. Piromlertamorn W. Objective: To study the effect of embryo density and microdrop volume on mouse two-cell embryo development. Design: Experimental study. Setting: Assisted conception laboratory. Animal(s): Two-cell mouse embryos (n = 1511). Intervention(s): One, five, 10, or 15 embryos were cultured in 10-μL drops of cleavage medium. In the second study, embryos were cultured singly in 0.5-, 1-, 2-, 5-, and 10-μL drops. Finally, they were cultured in pair in 0.5-, 1-, and 2-μL drops. After 48 hours, embryos were transferred into blastocyst medium for an additional 24 hours. Main Outcome Measure(s): Cleavage and blastocyst formation and inner cell mass (ICM) and trophectoderm (TE) cell numbers. Result(s): No differences in cleavage or blastocyst formation were found in different groups in experiment 1, 2, or 3. Embryos cultured singly had fewer ICM and TE cells than those cultured in groups. Embryos cultured singly in 0.5 μL had fewer TE cells than those in 10 μL, but had insignificant difference in the ICM. Duo culture in 0.5-2 μL appeared to give the same results as group culture in 10-μL drops. Conclusion(s): Group culture is preferred when using sequential media. Beneficial effects cannot be mimicked by volume reduction in single-embryo culture. © 2011 American Society for Reproductive Medicine, Published by Elsevier Inc. 2014-08-30T02:25:15Z 2014-08-30T02:25:15Z 2011 Article 150282 10.1016/j.fertnstert.2010.05.005 20561617 FESTA http://www.scopus.com/inward/record.url?eid=2-s2.0-79952448537&partnerID=40&md5=f919357c08b79d77d1302efa2ecf5d19 http://www.ncbi.nlm.nih.gov/pubmed/20561617 http://cmuir.cmu.ac.th/handle/6653943832/2684 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Objective: To study the effect of embryo density and microdrop volume on mouse two-cell embryo development. Design: Experimental study. Setting: Assisted conception laboratory. Animal(s): Two-cell mouse embryos (n = 1511). Intervention(s): One, five, 10, or 15 embryos were cultured in 10-μL drops of cleavage medium. In the second study, embryos were cultured singly in 0.5-, 1-, 2-, 5-, and 10-μL drops. Finally, they were cultured in pair in 0.5-, 1-, and 2-μL drops. After 48 hours, embryos were transferred into blastocyst medium for an additional 24 hours. Main Outcome Measure(s): Cleavage and blastocyst formation and inner cell mass (ICM) and trophectoderm (TE) cell numbers. Result(s): No differences in cleavage or blastocyst formation were found in different groups in experiment 1, 2, or 3. Embryos cultured singly had fewer ICM and TE cells than those cultured in groups. Embryos cultured singly in 0.5 μL had fewer TE cells than those in 10 μL, but had insignificant difference in the ICM. Duo culture in 0.5-2 μL appeared to give the same results as group culture in 10-μL drops. Conclusion(s): Group culture is preferred when using sequential media. Beneficial effects cannot be mimicked by volume reduction in single-embryo culture. © 2011 American Society for Reproductive Medicine, Published by Elsevier Inc.
format Article
author Vutyavanich T.
Saeng-Anan U.
Sirisukkasem S.
Piromlertamorn W.
spellingShingle Vutyavanich T.
Saeng-Anan U.
Sirisukkasem S.
Piromlertamorn W.
Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos
author_facet Vutyavanich T.
Saeng-Anan U.
Sirisukkasem S.
Piromlertamorn W.
author_sort Vutyavanich T.
title Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos
title_short Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos
title_full Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos
title_fullStr Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos
title_full_unstemmed Effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos
title_sort effect of embryo density and microdrop volume on the blastocyst development of mouse two-cell embryos
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-79952448537&partnerID=40&md5=f919357c08b79d77d1302efa2ecf5d19
http://www.ncbi.nlm.nih.gov/pubmed/20561617
http://cmuir.cmu.ac.th/handle/6653943832/2684
_version_ 1681419905299120128