Application of nested PCR to detect Penicillium marneffei in serum samples
We previously reported a nested PCR assay for specific identification of 18S ribosomal DNA of Penicillium marneffei. In this study, the assay was used to detect the DNA of P. marneffei in serum samples. Sensitivity of the test was 4 pg/l and 0.4 fg/l when the cycle numbers used for nested reactions...
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2014
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th-cmuir.6653943832-27332014-08-30T02:25:19Z Application of nested PCR to detect Penicillium marneffei in serum samples Pongpom M. Sirisanthana T. Vanittanakom N. We previously reported a nested PCR assay for specific identification of 18S ribosomal DNA of Penicillium marneffei. In this study, the assay was used to detect the DNA of P. marneffei in serum samples. Sensitivity of the test was 4 pg/l and 0.4 fg/l when the cycle numbers used for nested reactions were 15 and 30, respectively. Twenty four out of 35 sera (68.6%) collected from patients with culture confirmed penicilliosis marneffei were positive, while normal healthy and non-P. marneffei infected HIV-positive sera were negative. The results suggested that the assay could be applied for the diagnosis of infections due to P. marneffei. © 2009 ISHAM. 2014-08-30T02:25:19Z 2014-08-30T02:25:19Z 2009 Article 13693786 10.1080/13693780802484875 19353372 MEMYF http://www.scopus.com/inward/record.url?eid=2-s2.0-70749124150&partnerID=40&md5=da302d91538710ba11ef939faff2cf89 http://www.ncbi.nlm.nih.gov/pubmed/19353372 http://cmuir.cmu.ac.th/handle/6653943832/2733 English |
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We previously reported a nested PCR assay for specific identification of 18S ribosomal DNA of Penicillium marneffei. In this study, the assay was used to detect the DNA of P. marneffei in serum samples. Sensitivity of the test was 4 pg/l and 0.4 fg/l when the cycle numbers used for nested reactions were 15 and 30, respectively. Twenty four out of 35 sera (68.6%) collected from patients with culture confirmed penicilliosis marneffei were positive, while normal healthy and non-P. marneffei infected HIV-positive sera were negative. The results suggested that the assay could be applied for the diagnosis of infections due to P. marneffei. © 2009 ISHAM. |
| format |
Article |
| author |
Pongpom M. Sirisanthana T. Vanittanakom N. |
| spellingShingle |
Pongpom M. Sirisanthana T. Vanittanakom N. Application of nested PCR to detect Penicillium marneffei in serum samples |
| author_facet |
Pongpom M. Sirisanthana T. Vanittanakom N. |
| author_sort |
Pongpom M. |
| title |
Application of nested PCR to detect Penicillium marneffei in serum samples |
| title_short |
Application of nested PCR to detect Penicillium marneffei in serum samples |
| title_full |
Application of nested PCR to detect Penicillium marneffei in serum samples |
| title_fullStr |
Application of nested PCR to detect Penicillium marneffei in serum samples |
| title_full_unstemmed |
Application of nested PCR to detect Penicillium marneffei in serum samples |
| title_sort |
application of nested pcr to detect penicillium marneffei in serum samples |
| publishDate |
2014 |
| url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-70749124150&partnerID=40&md5=da302d91538710ba11ef939faff2cf89 http://www.ncbi.nlm.nih.gov/pubmed/19353372 http://cmuir.cmu.ac.th/handle/6653943832/2733 |
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