A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait

• Main Authors: Khonyoung S., Kradtap Hartwell S., Jakmunee J., Lapanantnoppakhun S., Sanguansermsri T., Grudpan K.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-68249089807&partnerID=40&md5=27a27aaa887591994b933d19034b261e; http://www.ncbi.nlm.nih.gov/pubmed/19531894; http://cmuir.cmu.ac.th/handle/6653943832/2845
• Institution: Chiang Mai University

Simple instrumentation and procedure with stopped flow system coupled to hydrodynamic injection and spectrophotometric detection were developed for automatic osmotic fragility test (OFT). OFT is a test for abnormal red blood cells based on the kinetics of their rupturing in a hypotonic saline solution. A portion of red cells was merged on-line with a hypotonic saline solution. They were mixed while flowing into the detector and were stopped for a short period for continuously monitoring the change of turbidity based on a transmission signal. In this work, a possible application of the system for screening of beta-thalassemia trait is demonstrated. Descriptions of instrumentation development and parameter optimization are presented. The system offers advantages over the conventional batch-wise OFT in terms of automation, precision, analysis time and sample volume. 2009 © The Japan Society for Analytical Chemistry.