Sensitivity and specificity of mean corpuscular hemoglobin (MCH): For screening alpha-thalassemia-1 trait and beta-thalassemia trait

Objective: To evaluate sensitivity, specificity, and positive and negative predictive value of mean corpuscular hemoglobin (MCH) for screening alpha-thalassemia-1 trait and beta-thalassemia trait Material and Method: Descriptive analysis, diagnostic test, was conducted on 396 pregnant women attendin...

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Main Authors: Pranpanus S., Sirichotiyakul S., Srisupundit K., Tongsong T.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-67650450528&partnerID=40&md5=7cbf9e14d959a446e2f4a1f19a21c293
http://www.ncbi.nlm.nih.gov/pubmed/19530577
http://cmuir.cmu.ac.th/handle/6653943832/2849
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-28492014-08-30T02:25:27Z Sensitivity and specificity of mean corpuscular hemoglobin (MCH): For screening alpha-thalassemia-1 trait and beta-thalassemia trait Pranpanus S. Sirichotiyakul S. Srisupundit K. Tongsong T. Objective: To evaluate sensitivity, specificity, and positive and negative predictive value of mean corpuscular hemoglobin (MCH) for screening alpha-thalassemia-1 trait and beta-thalassemia trait Material and Method: Descriptive analysis, diagnostic test, was conducted on 396 pregnant women attending the antenatal clinic between September 2007 and June 2008. Blood samples were collected from pregnant women after counseling and getting informed consent. MCH was measured in all samples by automated hematology analyzer. Determination of HbA2 level for diagnosis of beta-thalassmia trait and PCR for alphat-halassemia-1 gene (SEA type) were performed in all cases as a gold standard. The data were collected and analyzed for sensitivity, specificity, and positive and negative predictive value of MCH for screening alpha-thalassemia-1 trait and beta-thalassemia trait. Results: Based on the ROC curve, the best cut-off level of MCH in predicting the thalassemia carriers was 26.5 picrograms. Positive MCH (< 26.5 picrograms) gave the sensitivity of 95.2% and specificity of 82.3% in screening alpha-thalassemia-1 trait and beta-thalassemia trait. The positive predictive value and negative predictive value were 40.4% and 99.3% respectively. Conclusion: MCH is a good tool for screening alpha-thalassemia-1 trait and beta-thalassemia trait during pregnancy because of its simplicity, low cost, (when determined as a part of complete blood count), and high sensitivity. 2014-08-30T02:25:27Z 2014-08-30T02:25:27Z 2009 Article 01252208 19530577 JMTHB http://www.scopus.com/inward/record.url?eid=2-s2.0-67650450528&partnerID=40&md5=7cbf9e14d959a446e2f4a1f19a21c293 http://www.ncbi.nlm.nih.gov/pubmed/19530577 http://cmuir.cmu.ac.th/handle/6653943832/2849 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Objective: To evaluate sensitivity, specificity, and positive and negative predictive value of mean corpuscular hemoglobin (MCH) for screening alpha-thalassemia-1 trait and beta-thalassemia trait Material and Method: Descriptive analysis, diagnostic test, was conducted on 396 pregnant women attending the antenatal clinic between September 2007 and June 2008. Blood samples were collected from pregnant women after counseling and getting informed consent. MCH was measured in all samples by automated hematology analyzer. Determination of HbA2 level for diagnosis of beta-thalassmia trait and PCR for alphat-halassemia-1 gene (SEA type) were performed in all cases as a gold standard. The data were collected and analyzed for sensitivity, specificity, and positive and negative predictive value of MCH for screening alpha-thalassemia-1 trait and beta-thalassemia trait. Results: Based on the ROC curve, the best cut-off level of MCH in predicting the thalassemia carriers was 26.5 picrograms. Positive MCH (< 26.5 picrograms) gave the sensitivity of 95.2% and specificity of 82.3% in screening alpha-thalassemia-1 trait and beta-thalassemia trait. The positive predictive value and negative predictive value were 40.4% and 99.3% respectively. Conclusion: MCH is a good tool for screening alpha-thalassemia-1 trait and beta-thalassemia trait during pregnancy because of its simplicity, low cost, (when determined as a part of complete blood count), and high sensitivity.
format Article
author Pranpanus S.
Sirichotiyakul S.
Srisupundit K.
Tongsong T.
spellingShingle Pranpanus S.
Sirichotiyakul S.
Srisupundit K.
Tongsong T.
Sensitivity and specificity of mean corpuscular hemoglobin (MCH): For screening alpha-thalassemia-1 trait and beta-thalassemia trait
author_facet Pranpanus S.
Sirichotiyakul S.
Srisupundit K.
Tongsong T.
author_sort Pranpanus S.
title Sensitivity and specificity of mean corpuscular hemoglobin (MCH): For screening alpha-thalassemia-1 trait and beta-thalassemia trait
title_short Sensitivity and specificity of mean corpuscular hemoglobin (MCH): For screening alpha-thalassemia-1 trait and beta-thalassemia trait
title_full Sensitivity and specificity of mean corpuscular hemoglobin (MCH): For screening alpha-thalassemia-1 trait and beta-thalassemia trait
title_fullStr Sensitivity and specificity of mean corpuscular hemoglobin (MCH): For screening alpha-thalassemia-1 trait and beta-thalassemia trait
title_full_unstemmed Sensitivity and specificity of mean corpuscular hemoglobin (MCH): For screening alpha-thalassemia-1 trait and beta-thalassemia trait
title_sort sensitivity and specificity of mean corpuscular hemoglobin (mch): for screening alpha-thalassemia-1 trait and beta-thalassemia trait
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-67650450528&partnerID=40&md5=7cbf9e14d959a446e2f4a1f19a21c293
http://www.ncbi.nlm.nih.gov/pubmed/19530577
http://cmuir.cmu.ac.th/handle/6653943832/2849
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