Genotypic distribution of hepatitis c virus in voluntary blood donors of northern Thailand

The purpose of this study was to determine the prevalence and distribution of HCV genotypes among voluntary blood donors in northern Thailand. From 1998 to 2000,167 serum samples which tested positive for anti-HCV antibodies in the screening of voluntary blood donors from 5 provinces in northern Tha...

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Main Authors: Jutavijittum P., Jiviriyawat Y., Yousukh A., Pantip C., Maneekarn N., Toriyama K.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-67650228091&partnerID=40&md5=b8f71179aeb0e8d79a854083d2c29efc
http://www.ncbi.nlm.nih.gov/pubmed/19842432
http://cmuir.cmu.ac.th/handle/6653943832/2860
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-28602014-08-30T02:25:28Z Genotypic distribution of hepatitis c virus in voluntary blood donors of northern Thailand Jutavijittum P. Jiviriyawat Y. Yousukh A. Pantip C. Maneekarn N. Toriyama K. The purpose of this study was to determine the prevalence and distribution of HCV genotypes among voluntary blood donors in northern Thailand. From 1998 to 2000,167 serum samples which tested positive for anti-HCV antibodies in the screening of voluntary blood donors from 5 provinces in northern Thailand were selected for genotyping. Viral RNA was extracted from the sera. The core-El region of the HCV- RNA genome was amplified using a OneStep RT-PCR kit. The core-El amplicon was sequenced and the HCV genotype was assigned by comparing with the reference sequences available in the GenBank database. Of 167 anti-HCV positive serum samples, 126 (75.4%) contained HCV RNA as detected by PCR. HCV genotype 3 was the most predominant genotype (39.6%), of which 33.3% belonged to genotype 3a and 6.3% to 3b. Genotype 6 was detected in 31%, and genotype 1 was detected in 27.8%. Of the genotype 1 isolates, 14.3% were la, 12.7% were lb, and 0.8% were lc. Two HCV isolates detected in the present study were untypeable. About 75% of anti-HCV positive blood donors had chronic HCV infection. In northern Thailand, genotype 3a was the most predominant genotype, while genotype 6, la and lb were also commonly found. The genotypic distribution of HCV isolates from various regions of Thailand were more or less similar. Nevertheless, in this study, the prevalence of HCV genotype 6 (31%) was higher than previously reported by others (8-18%). Phylogenetic analysis of the HCV isolates detected in the present study was also performed. 2014-08-30T02:25:28Z 2014-08-30T02:25:28Z 2009 Article 01251562 19842432 http://www.scopus.com/inward/record.url?eid=2-s2.0-67650228091&partnerID=40&md5=b8f71179aeb0e8d79a854083d2c29efc http://www.ncbi.nlm.nih.gov/pubmed/19842432 http://cmuir.cmu.ac.th/handle/6653943832/2860 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description The purpose of this study was to determine the prevalence and distribution of HCV genotypes among voluntary blood donors in northern Thailand. From 1998 to 2000,167 serum samples which tested positive for anti-HCV antibodies in the screening of voluntary blood donors from 5 provinces in northern Thailand were selected for genotyping. Viral RNA was extracted from the sera. The core-El region of the HCV- RNA genome was amplified using a OneStep RT-PCR kit. The core-El amplicon was sequenced and the HCV genotype was assigned by comparing with the reference sequences available in the GenBank database. Of 167 anti-HCV positive serum samples, 126 (75.4%) contained HCV RNA as detected by PCR. HCV genotype 3 was the most predominant genotype (39.6%), of which 33.3% belonged to genotype 3a and 6.3% to 3b. Genotype 6 was detected in 31%, and genotype 1 was detected in 27.8%. Of the genotype 1 isolates, 14.3% were la, 12.7% were lb, and 0.8% were lc. Two HCV isolates detected in the present study were untypeable. About 75% of anti-HCV positive blood donors had chronic HCV infection. In northern Thailand, genotype 3a was the most predominant genotype, while genotype 6, la and lb were also commonly found. The genotypic distribution of HCV isolates from various regions of Thailand were more or less similar. Nevertheless, in this study, the prevalence of HCV genotype 6 (31%) was higher than previously reported by others (8-18%). Phylogenetic analysis of the HCV isolates detected in the present study was also performed.
format Article
author Jutavijittum P.
Jiviriyawat Y.
Yousukh A.
Pantip C.
Maneekarn N.
Toriyama K.
spellingShingle Jutavijittum P.
Jiviriyawat Y.
Yousukh A.
Pantip C.
Maneekarn N.
Toriyama K.
Genotypic distribution of hepatitis c virus in voluntary blood donors of northern Thailand
author_facet Jutavijittum P.
Jiviriyawat Y.
Yousukh A.
Pantip C.
Maneekarn N.
Toriyama K.
author_sort Jutavijittum P.
title Genotypic distribution of hepatitis c virus in voluntary blood donors of northern Thailand
title_short Genotypic distribution of hepatitis c virus in voluntary blood donors of northern Thailand
title_full Genotypic distribution of hepatitis c virus in voluntary blood donors of northern Thailand
title_fullStr Genotypic distribution of hepatitis c virus in voluntary blood donors of northern Thailand
title_full_unstemmed Genotypic distribution of hepatitis c virus in voluntary blood donors of northern Thailand
title_sort genotypic distribution of hepatitis c virus in voluntary blood donors of northern thailand
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-67650228091&partnerID=40&md5=b8f71179aeb0e8d79a854083d2c29efc
http://www.ncbi.nlm.nih.gov/pubmed/19842432
http://cmuir.cmu.ac.th/handle/6653943832/2860
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