Chondrogenic properties of primary human chondrocytes culture in hyaluronic acid treated gelatin scaffold
Objective: To study the possibility of primary human chondrocytes culture in gelatin scaffold and the effects of exogenous HA on chondrocyte differentiation and synthesis of the hyaline-like extracellular matrix. Material and Method: Cartilage tissue was engineered by using primary human chondrocyte...
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th-cmuir.6653943832-28762014-08-30T02:25:30Z Chondrogenic properties of primary human chondrocytes culture in hyaluronic acid treated gelatin scaffold Pruksakorn D. Khamwaen N. Pothacharoen P. Arpornchayanon O. Rojanasthien S. Kongtawelert P. Objective: To study the possibility of primary human chondrocytes culture in gelatin scaffold and the effects of exogenous HA on chondrocyte differentiation and synthesis of the hyaline-like extracellular matrix. Material and Method: Cartilage tissue was engineered by using primary human chondrocytes with HA-treated gelatin scaffolds and gelatin scaffolds. The chondrogenic properties were monitored for chondrocyte proliferation, adhesion, and hyaline-like extracellular matrix production in both groups. The results were compared to each other. Results: Chondrocyte proliferation, adhesive activity, and new HA production were significantly increased in HA-treated gelatin scaffold (p < 0.05). Immuno histochemistry for WF6 epitope demonstrated the higher quality of hyaline-like extracellular matrix production. Moreover, the scanning electron micrograph showed a higher filling of extracellular matrix in the pore of scaffold of HA-treated gelatin scaffold than that in non-HA treated scaffold. Conclusion: The present study demonstrated the possible role of commercial gelatin-based scaffold in cartilage tissue engineering. It also demonstrated that exogenous HA-treated scaffold provides positive effects for chondrocytes. 2014-08-30T02:25:30Z 2014-08-30T02:25:30Z 2009 Article 01252208 19374298 JMTHB http://www.scopus.com/inward/record.url?eid=2-s2.0-65649140009&partnerID=40&md5=cb550fb8a138100a0d44d355615e708f http://www.ncbi.nlm.nih.gov/pubmed/19374298 http://cmuir.cmu.ac.th/handle/6653943832/2876 English |
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Objective: To study the possibility of primary human chondrocytes culture in gelatin scaffold and the effects of exogenous HA on chondrocyte differentiation and synthesis of the hyaline-like extracellular matrix. Material and Method: Cartilage tissue was engineered by using primary human chondrocytes with HA-treated gelatin scaffolds and gelatin scaffolds. The chondrogenic properties were monitored for chondrocyte proliferation, adhesion, and hyaline-like extracellular matrix production in both groups. The results were compared to each other. Results: Chondrocyte proliferation, adhesive activity, and new HA production were significantly increased in HA-treated gelatin scaffold (p < 0.05). Immuno histochemistry for WF6 epitope demonstrated the higher quality of hyaline-like extracellular matrix production. Moreover, the scanning electron micrograph showed a higher filling of extracellular matrix in the pore of scaffold of HA-treated gelatin scaffold than that in non-HA treated scaffold. Conclusion: The present study demonstrated the possible role of commercial gelatin-based scaffold in cartilage tissue engineering. It also demonstrated that exogenous HA-treated scaffold provides positive effects for chondrocytes. |
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Article |
author |
Pruksakorn D. Khamwaen N. Pothacharoen P. Arpornchayanon O. Rojanasthien S. Kongtawelert P. |
spellingShingle |
Pruksakorn D. Khamwaen N. Pothacharoen P. Arpornchayanon O. Rojanasthien S. Kongtawelert P. Chondrogenic properties of primary human chondrocytes culture in hyaluronic acid treated gelatin scaffold |
author_facet |
Pruksakorn D. Khamwaen N. Pothacharoen P. Arpornchayanon O. Rojanasthien S. Kongtawelert P. |
author_sort |
Pruksakorn D. |
title |
Chondrogenic properties of primary human chondrocytes culture in hyaluronic acid treated gelatin scaffold |
title_short |
Chondrogenic properties of primary human chondrocytes culture in hyaluronic acid treated gelatin scaffold |
title_full |
Chondrogenic properties of primary human chondrocytes culture in hyaluronic acid treated gelatin scaffold |
title_fullStr |
Chondrogenic properties of primary human chondrocytes culture in hyaluronic acid treated gelatin scaffold |
title_full_unstemmed |
Chondrogenic properties of primary human chondrocytes culture in hyaluronic acid treated gelatin scaffold |
title_sort |
chondrogenic properties of primary human chondrocytes culture in hyaluronic acid treated gelatin scaffold |
publishDate |
2014 |
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http://www.scopus.com/inward/record.url?eid=2-s2.0-65649140009&partnerID=40&md5=cb550fb8a138100a0d44d355615e708f http://www.ncbi.nlm.nih.gov/pubmed/19374298 http://cmuir.cmu.ac.th/handle/6653943832/2876 |
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