Development of genotype-specific primers for differentiation of genotypes A and B of Aichi viruses

• Main Authors: Pham N.T.K., Trinh Q.D., Nguyen T.A., Dey S.K., Phan T.G., Hoang L.P., Khamrin P., Maneekarn N., Okitsu S., Mizuguchi M., Ushijima H.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-59749093977&partnerID=40&md5=ffd4a08d3f15287a7c00d731a7d2055f; http://cmuir.cmu.ac.th/handle/6653943832/2894
• Institution: Chiang Mai University

A nested polymerase chain reaction method using genotype-specific primers based on the capsid gene was developed to differentiate between genotypes A and B of Aichi viruses. Results of the study showed that the PCR using newly designed genotype-specific primers could generate appropriate PCR products from all 17 samples tested, the newly developed primers could differentiate genotype A from genotype B, and all matched those obtained by nucleotide sequencing of the capsid regions. The nested PCR method using genotype-specific primers is useful and can be used for genotyping of Aichi viruses isolated from epidemiological studies. © 2008 Elsevier B.V. All rights reserved.