Reproductive organ of blow fly, Chrysomya megacephala (diptera: Calliphoridae): Ultrastructural of testis

• Main Authors: Sukontason K.L., Chaiwong T., Chaisri U., Kurahashi H., Sanford M., Sukontason K.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-80053464389&partnerID=40&md5=42f0d64d0d7962e1662e78adde92ca98; http://www.ncbi.nlm.nih.gov/pubmed/21845212; http://cmuir.cmu.ac.th/handle/6653943832/2986
• Institution: Chiang Mai University

This work presents the ultrastructure of testis of the medically important blow fly, Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) using light microscopy and electron microscopy. Reproductive organ of males was dissected to determine the testis in the pupal stage, 3-day-old flies and 7-day-old flies and observed under scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM displayed a smooth surface which is occasionally penetrated by tracheoles. TEM of the testis in the pupal stage presents the thick testis wall covering underdeveloped cells containing a variable size of an electron-dense globule. For the 3-day-old males, the testicular wall is formed by an external layer, a peritoneal sheath, a muscular layer, a basement membrane, and a follicular epithelium. Follicular epithelium presented developing spermatozoa. Regarding the 7-day-old males, development of spermatozoa is apparent, displaying nucleus, centriolar adjunct, axoneme, and mitochondrial derivatives, with the 9 + 9 + 2 microtubule pattern of axoneme. © 2011 Kabkaew L. Sukontason et al.