Chronological observations of intestinal histopathology in rats (Rattus norvegicus) infected with Centrocestus caninus

Intestinal pathological enzyme activity changes were studied chronologically in rats after Centrocestus caninus infection. A single inoculation of 300 metacercariae isolated from the gills of goldfish (Carassius auratus), was orally administered to male rats (n = 15). Uninfected animals were used as...

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Main Authors: Saenphet S., Wongsawad C., Saenphet K., Rojanapaibul A., Vanittanakom P., Chai JY.
Format: Article
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/3146
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-31462014-08-30T02:25:49Z Chronological observations of intestinal histopathology in rats (Rattus norvegicus) infected with Centrocestus caninus Saenphet S. Wongsawad C. Saenphet K. Rojanapaibul A. Vanittanakom P. Chai JY. Intestinal pathological enzyme activity changes were studied chronologically in rats after Centrocestus caninus infection. A single inoculation of 300 metacercariae isolated from the gills of goldfish (Carassius auratus), was orally administered to male rats (n = 15). Uninfected animals were used as controls (n = 5). At days 3, 7, 14, 21, and 28 post-infection (PI), three infected rats, and one from each control group, were sacrificed. The duodenum, jejunum, and ileum were removed separately and fixed in 10% formalin and 10% cold formal calcium solution for histopathological and alkaline phosphatase activity investigations, respectively. The worms were found intruded into the intervillous space of the mucosa and the mucosa showed villous atrophy, crypt hyperplasia and stromal inflammation with inflammatory cell accumulations. Alkaline phosphatase (ALP) activity also showed retardation. However, it seemed that these phenomena would return to normal at the end of the experiment. It can be concluded, from our data, that C. caninus could cause mild histopathological alterations and reduce ALP activity in the small intestines. 2014-08-30T02:25:49Z 2014-08-30T02:25:49Z 2006 Journal Article 0125-1562 17547056 http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/3146 eng
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Intestinal pathological enzyme activity changes were studied chronologically in rats after Centrocestus caninus infection. A single inoculation of 300 metacercariae isolated from the gills of goldfish (Carassius auratus), was orally administered to male rats (n = 15). Uninfected animals were used as controls (n = 5). At days 3, 7, 14, 21, and 28 post-infection (PI), three infected rats, and one from each control group, were sacrificed. The duodenum, jejunum, and ileum were removed separately and fixed in 10% formalin and 10% cold formal calcium solution for histopathological and alkaline phosphatase activity investigations, respectively. The worms were found intruded into the intervillous space of the mucosa and the mucosa showed villous atrophy, crypt hyperplasia and stromal inflammation with inflammatory cell accumulations. Alkaline phosphatase (ALP) activity also showed retardation. However, it seemed that these phenomena would return to normal at the end of the experiment. It can be concluded, from our data, that C. caninus could cause mild histopathological alterations and reduce ALP activity in the small intestines.
format Article
author Saenphet S.
Wongsawad C.
Saenphet K.
Rojanapaibul A.
Vanittanakom P.
Chai JY.
spellingShingle Saenphet S.
Wongsawad C.
Saenphet K.
Rojanapaibul A.
Vanittanakom P.
Chai JY.
Chronological observations of intestinal histopathology in rats (Rattus norvegicus) infected with Centrocestus caninus
author_facet Saenphet S.
Wongsawad C.
Saenphet K.
Rojanapaibul A.
Vanittanakom P.
Chai JY.
author_sort Saenphet S.
title Chronological observations of intestinal histopathology in rats (Rattus norvegicus) infected with Centrocestus caninus
title_short Chronological observations of intestinal histopathology in rats (Rattus norvegicus) infected with Centrocestus caninus
title_full Chronological observations of intestinal histopathology in rats (Rattus norvegicus) infected with Centrocestus caninus
title_fullStr Chronological observations of intestinal histopathology in rats (Rattus norvegicus) infected with Centrocestus caninus
title_full_unstemmed Chronological observations of intestinal histopathology in rats (Rattus norvegicus) infected with Centrocestus caninus
title_sort chronological observations of intestinal histopathology in rats (rattus norvegicus) infected with centrocestus caninus
publishDate 2014
url http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/3146
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