Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique

Genetic relationship of 36 samples from 32 species of the genus Phalaenopsis and related genera was evaluated based on RAPD technique using 20 decamer primers. Six primers that were OPAK 10, OPD 03, OPF 01, OPF 02, OPF 09 and OPF 14 showed polymorphic DNA bands with high resolution and produced 78 p...

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Main Authors: Taywiya P., Bundithya W., Potapohn N.
Format: Conference or Workshop Item
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-61449093941&partnerID=40&md5=3f7d17008e2f3dee5bae3bd4e875fdb5
http://cmuir.cmu.ac.th/handle/6653943832/319
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-3192014-08-29T07:31:35Z Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique Taywiya P. Bundithya W. Potapohn N. Genetic relationship of 36 samples from 32 species of the genus Phalaenopsis and related genera was evaluated based on RAPD technique using 20 decamer primers. Six primers that were OPAK 10, OPD 03, OPF 01, OPF 02, OPF 09 and OPF 14 showed polymorphic DNA bands with high resolution and produced 78 polymorphic bands ranged in size from 223 to 2,300 bp. Genetic relationship was determined by UPGMA cluster analysis, using the Program NTSYS-PC version 2.01. Cluster analysis of six primer combinations could distinguish and divided the genus Phalaenopsis and related genera into 11 groups, which were correspondent to the analysis of genus Phalaenopsis by morphological characteristics. The genera Doritis and Kingidium were genetically distant from the genus Phalaenopsis. 2014-08-29T07:31:35Z 2014-08-29T07:31:35Z 2008 Conference Paper 05677572 http://www.scopus.com/inward/record.url?eid=2-s2.0-61449093941&partnerID=40&md5=3f7d17008e2f3dee5bae3bd4e875fdb5 http://cmuir.cmu.ac.th/handle/6653943832/319 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Genetic relationship of 36 samples from 32 species of the genus Phalaenopsis and related genera was evaluated based on RAPD technique using 20 decamer primers. Six primers that were OPAK 10, OPD 03, OPF 01, OPF 02, OPF 09 and OPF 14 showed polymorphic DNA bands with high resolution and produced 78 polymorphic bands ranged in size from 223 to 2,300 bp. Genetic relationship was determined by UPGMA cluster analysis, using the Program NTSYS-PC version 2.01. Cluster analysis of six primer combinations could distinguish and divided the genus Phalaenopsis and related genera into 11 groups, which were correspondent to the analysis of genus Phalaenopsis by morphological characteristics. The genera Doritis and Kingidium were genetically distant from the genus Phalaenopsis.
format Conference or Workshop Item
author Taywiya P.
Bundithya W.
Potapohn N.
spellingShingle Taywiya P.
Bundithya W.
Potapohn N.
Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique
author_facet Taywiya P.
Bundithya W.
Potapohn N.
author_sort Taywiya P.
title Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique
title_short Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique
title_full Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique
title_fullStr Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique
title_full_unstemmed Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique
title_sort analysis of genetic relationship of the genus phalaenopsis by rapd technique
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-61449093941&partnerID=40&md5=3f7d17008e2f3dee5bae3bd4e875fdb5
http://cmuir.cmu.ac.th/handle/6653943832/319
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