Western immunoblot analysis of protein antigens of Penicillium marneffei

Protein antigens of Penicillium marneffei prepared during the yeast and mould phases of in vitro growth were analyzed by gel electrophoresis and immunoblot assay. More than 20 yeast phase proteins were detected by Coomassie staining; among these, at least 10 reacted with IgG in the pooled sera of 28...

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Main Authors: Vanittanakom N., Mekaprateep M., Sittisombut N., Supparatpinyo K., Kanjanasthiti P., Nelson KE., Sirisanthana T.
Format: Article
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/3439
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-34392014-08-30T02:26:07Z Western immunoblot analysis of protein antigens of Penicillium marneffei Vanittanakom N. Mekaprateep M. Sittisombut N. Supparatpinyo K. Kanjanasthiti P. Nelson KE. Sirisanthana T. Protein antigens of Penicillium marneffei prepared during the yeast and mould phases of in vitro growth were analyzed by gel electrophoresis and immunoblot assay. More than 20 yeast phase proteins were detected by Coomassie staining; among these, at least 10 reacted with IgG in the pooled sera of 28 AIDS patients with penicilliosis. Four immunogenic proteins of 200, 88, 54 and 50 kDa were produced in large quantity during the deceleration and early stationary phases of growth. When these proteins were reacted with individual sera derived from 33 AIDS patients with penicilliosis, reactivities to the 200, 88, 54 and 50 kDa protein were detected in 72.7, 93.9, 60.6 and 57.6%, respectively. The bands of 88, 54 and 50 kDa gave strong reactions with about a half of serum samples. In one serum derived from an AIDS patient, reactivities to the 54 and 50 kDa proteins could be strongly detected two months before the definite diagnosis by fungal culture. Protein components from the mould form were of lower yield and gave weaker signal in immunoblot analysis. These results indicate that at least two yeast-phase immunoreactive proteins (54 and 50 kDa) are relatively specific to the P. marneffei infection, thereby suggesting its potential for clinical application to the diagnosis of this emerging disease. 2014-08-30T02:26:07Z 2014-08-30T02:26:07Z Journal Article 0268-1218 9147272 http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/3439 eng
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Protein antigens of Penicillium marneffei prepared during the yeast and mould phases of in vitro growth were analyzed by gel electrophoresis and immunoblot assay. More than 20 yeast phase proteins were detected by Coomassie staining; among these, at least 10 reacted with IgG in the pooled sera of 28 AIDS patients with penicilliosis. Four immunogenic proteins of 200, 88, 54 and 50 kDa were produced in large quantity during the deceleration and early stationary phases of growth. When these proteins were reacted with individual sera derived from 33 AIDS patients with penicilliosis, reactivities to the 200, 88, 54 and 50 kDa protein were detected in 72.7, 93.9, 60.6 and 57.6%, respectively. The bands of 88, 54 and 50 kDa gave strong reactions with about a half of serum samples. In one serum derived from an AIDS patient, reactivities to the 54 and 50 kDa proteins could be strongly detected two months before the definite diagnosis by fungal culture. Protein components from the mould form were of lower yield and gave weaker signal in immunoblot analysis. These results indicate that at least two yeast-phase immunoreactive proteins (54 and 50 kDa) are relatively specific to the P. marneffei infection, thereby suggesting its potential for clinical application to the diagnosis of this emerging disease.
format Article
author Vanittanakom N.
Mekaprateep M.
Sittisombut N.
Supparatpinyo K.
Kanjanasthiti P.
Nelson KE.
Sirisanthana T.
spellingShingle Vanittanakom N.
Mekaprateep M.
Sittisombut N.
Supparatpinyo K.
Kanjanasthiti P.
Nelson KE.
Sirisanthana T.
Western immunoblot analysis of protein antigens of Penicillium marneffei
author_facet Vanittanakom N.
Mekaprateep M.
Sittisombut N.
Supparatpinyo K.
Kanjanasthiti P.
Nelson KE.
Sirisanthana T.
author_sort Vanittanakom N.
title Western immunoblot analysis of protein antigens of Penicillium marneffei
title_short Western immunoblot analysis of protein antigens of Penicillium marneffei
title_full Western immunoblot analysis of protein antigens of Penicillium marneffei
title_fullStr Western immunoblot analysis of protein antigens of Penicillium marneffei
title_full_unstemmed Western immunoblot analysis of protein antigens of Penicillium marneffei
title_sort western immunoblot analysis of protein antigens of penicillium marneffei
publishDate 2014
url http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/3439
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