Effects of reactive oxygen species (ROS) modulators, TEMPOL and catalase, on methoxyacetaldehyde (MALD) -induced chromosome aberrations in Chinese hamster ovary (CHO)-AS52 cells

Methoxyacetaldehyde (MALD), a metabolite of 2-methoxyethanol, has been shown to be clastogenic and mutagenic in CHO-AS52 cells. PCR-based-deletion screening of MALD induced CHO-AS52 mutants indicates that MALD induces large deletion mutation. Since MALD has an aldehyde as its reactive functional gro...

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Bibliographic Details
Main Authors: Ratanavalachai TC., Au WW.
Format: Article
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/3444
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Institution: Chiang Mai University
Language: English
Description
Summary:Methoxyacetaldehyde (MALD), a metabolite of 2-methoxyethanol, has been shown to be clastogenic and mutagenic in CHO-AS52 cells. PCR-based-deletion screening of MALD induced CHO-AS52 mutants indicates that MALD induces large deletion mutation. Since MALD has an aldehyde as its reactive functional group, it can react with aldehyde oxidase to produce superoxide. The generation of these reactive oxygen species (superoxide, hydrogen peroxide and hydroxyl radical) may be the mechanism for genotoxicity of MALD. In the present study, TEMPOL and catalase which are ROS modulators were used to study the effects on MALD-induced chromosome damage in CHO-AS52 cells. The results showed that neither TEMPOL nor catalase can protect cells from MALD-induced chromosome aberrations. Therefore, the generation of reactive oxygen species may not be the primary mechanism of action of MALD.