Efficiency of the flotation method in the isolation of Penicillium marneffei from seeded soil

The efficiency of a modified flotation method for the isolation of Penicillium marneffei from soils has been evaluated. About 80% was recovered from sterilized soil freshly seeded with P. marneffei (100 conidia/1.5 g soil). When seeded non-sterile soil was used (at least 100 seeded conidia/1.5 g soi...

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Bibliographic Details
Main Authors: Vanittanakom N., Mekaprateep M., Sriburee P., Vanittanakom P., Khanjanasthiti P.
Format: Comparative Study
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/3472
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Institution: Chiang Mai University
Language: English
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Summary:The efficiency of a modified flotation method for the isolation of Penicillium marneffei from soils has been evaluated. About 80% was recovered from sterilized soil freshly seeded with P. marneffei (100 conidia/1.5 g soil). When seeded non-sterile soil was used (at least 100 seeded conidia/1.5 g soil), P. marneffei could be effectively recovered by employing a combination of the flotation method and the mouse inoculation method.