Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions
Mouse-tobacco hybrid calli, and complete plants producing mouse gamma-3 heavy and lambda light chains, have been generated by somatic cell fusions of mouse spleen cells and tobacco mesophyll protoplasts. Both gamma 3 and lambda chains were detected in hybrid calli and complete plants by enzyme-linke...
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2014
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th-cmuir.6653943832-34812014-08-30T02:34:55Z Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions Makonkawkeyoon S. Smitamana P. Hirunpetcharat C. Maneekarn N. Mouse-tobacco hybrid calli, and complete plants producing mouse gamma-3 heavy and lambda light chains, have been generated by somatic cell fusions of mouse spleen cells and tobacco mesophyll protoplasts. Both gamma 3 and lambda chains were detected in hybrid calli and complete plants by enzyme-linked immunosorbent assay, immunofluorescent staining, and Western blotting. When cellular DNA from hybrid tobacco mesophyll protoplasts was amplified by the polymerase chain reaction (PCR) using two pairs of gamma 3 chain DNA primers and one pair of lambda chain DNA primers, the PCR products contained gamma 3 and lambda chain DNAs, which could be detected by southern blotting and DNA hybridization, using specific synthetic oligonucleotide probes for gamma 3 and lambda respectively. In situ hybridization of hybrid tobacco mesophyll protoplasts with specific recombinant DNA probes of gamma 3 and lambda chains showed the presence of gamma 3 and lambda chain DNAs in the hybrid protoplasts. 2014-08-30T02:34:55Z 2014-08-30T02:34:55Z 1995 Journal Article 0014-4754 7843325 http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/3481 eng |
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Mouse-tobacco hybrid calli, and complete plants producing mouse gamma-3 heavy and lambda light chains, have been generated by somatic cell fusions of mouse spleen cells and tobacco mesophyll protoplasts. Both gamma 3 and lambda chains were detected in hybrid calli and complete plants by enzyme-linked immunosorbent assay, immunofluorescent staining, and Western blotting. When cellular DNA from hybrid tobacco mesophyll protoplasts was amplified by the polymerase chain reaction (PCR) using two pairs of gamma 3 chain DNA primers and one pair of lambda chain DNA primers, the PCR products contained gamma 3 and lambda chain DNAs, which could be detected by southern blotting and DNA hybridization, using specific synthetic oligonucleotide probes for gamma 3 and lambda respectively. In situ hybridization of hybrid tobacco mesophyll protoplasts with specific recombinant DNA probes of gamma 3 and lambda chains showed the presence of gamma 3 and lambda chain DNAs in the hybrid protoplasts. |
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Article |
author |
Makonkawkeyoon S. Smitamana P. Hirunpetcharat C. Maneekarn N. |
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Makonkawkeyoon S. Smitamana P. Hirunpetcharat C. Maneekarn N. Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions |
author_facet |
Makonkawkeyoon S. Smitamana P. Hirunpetcharat C. Maneekarn N. |
author_sort |
Makonkawkeyoon S. |
title |
Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions |
title_short |
Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions |
title_full |
Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions |
title_fullStr |
Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions |
title_full_unstemmed |
Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions |
title_sort |
production of mouse immunoglobulin g by a hybrid plant derived from tobacco-mouse cell fusions |
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2014 |
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http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/3481 |
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