Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions

Mouse-tobacco hybrid calli, and complete plants producing mouse gamma-3 heavy and lambda light chains, have been generated by somatic cell fusions of mouse spleen cells and tobacco mesophyll protoplasts. Both gamma 3 and lambda chains were detected in hybrid calli and complete plants by enzyme-linke...

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Main Authors: Makonkawkeyoon S., Smitamana P., Hirunpetcharat C., Maneekarn N.
Format: Article
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/3481
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-34812014-08-30T02:34:55Z Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions Makonkawkeyoon S. Smitamana P. Hirunpetcharat C. Maneekarn N. Mouse-tobacco hybrid calli, and complete plants producing mouse gamma-3 heavy and lambda light chains, have been generated by somatic cell fusions of mouse spleen cells and tobacco mesophyll protoplasts. Both gamma 3 and lambda chains were detected in hybrid calli and complete plants by enzyme-linked immunosorbent assay, immunofluorescent staining, and Western blotting. When cellular DNA from hybrid tobacco mesophyll protoplasts was amplified by the polymerase chain reaction (PCR) using two pairs of gamma 3 chain DNA primers and one pair of lambda chain DNA primers, the PCR products contained gamma 3 and lambda chain DNAs, which could be detected by southern blotting and DNA hybridization, using specific synthetic oligonucleotide probes for gamma 3 and lambda respectively. In situ hybridization of hybrid tobacco mesophyll protoplasts with specific recombinant DNA probes of gamma 3 and lambda chains showed the presence of gamma 3 and lambda chain DNAs in the hybrid protoplasts. 2014-08-30T02:34:55Z 2014-08-30T02:34:55Z 1995 Journal Article 0014-4754 7843325 http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/3481 eng
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Mouse-tobacco hybrid calli, and complete plants producing mouse gamma-3 heavy and lambda light chains, have been generated by somatic cell fusions of mouse spleen cells and tobacco mesophyll protoplasts. Both gamma 3 and lambda chains were detected in hybrid calli and complete plants by enzyme-linked immunosorbent assay, immunofluorescent staining, and Western blotting. When cellular DNA from hybrid tobacco mesophyll protoplasts was amplified by the polymerase chain reaction (PCR) using two pairs of gamma 3 chain DNA primers and one pair of lambda chain DNA primers, the PCR products contained gamma 3 and lambda chain DNAs, which could be detected by southern blotting and DNA hybridization, using specific synthetic oligonucleotide probes for gamma 3 and lambda respectively. In situ hybridization of hybrid tobacco mesophyll protoplasts with specific recombinant DNA probes of gamma 3 and lambda chains showed the presence of gamma 3 and lambda chain DNAs in the hybrid protoplasts.
format Article
author Makonkawkeyoon S.
Smitamana P.
Hirunpetcharat C.
Maneekarn N.
spellingShingle Makonkawkeyoon S.
Smitamana P.
Hirunpetcharat C.
Maneekarn N.
Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions
author_facet Makonkawkeyoon S.
Smitamana P.
Hirunpetcharat C.
Maneekarn N.
author_sort Makonkawkeyoon S.
title Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions
title_short Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions
title_full Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions
title_fullStr Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions
title_full_unstemmed Production of mouse immunoglobulin G by a hybrid plant derived from tobacco-mouse cell fusions
title_sort production of mouse immunoglobulin g by a hybrid plant derived from tobacco-mouse cell fusions
publishDate 2014
url http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/3481
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