The value of enzyme-linked immunosorbent assay (ELISA) for diagnosis of human trichinosis

• Main Authors: Morakote N., Khamboonruang C., Siriprasert V., Suphawitayanukul S., Marcanantachoti S., Thamasonthi W.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.ncbi.nlm.nih.gov/pubmed/3502482; http://cmuir.cmu.ac.th/handle/6653943832/3558
• Institution: Chiang Mai University

Sera were collected from proven cases of trichinosis and other helminthic infections and examined for IgG and IgM antibodies by an enzyme-linked immunosorbent assay (ELISA). IgG-ELISA was positive in 68.75, 100, and 100% of cases on 23rd, 50th, 57th day of infection while IgM-ELISA was positive in 12.50, 85.71, 93.33% respectively. In the group with parasitic infections other than trichinosis 20.93% were positive by IgG-ELISA and strong cross-reactivity was prominent with intestinal capillariasis. With IgM-ELISA, positivity in the group with other parasitic infections was 9.30%. It was suggested that sera should be examined not before 1 month after infection to achieve maximum sensitivity for serodiagnosis of human trichinosis.