Enhanced production of functional extracellular single chain variable fragment against HIV-1 matrix protein from escherichia coli by sequential simplex optimization

• Main Authors: Intachai K., Singboottra P., Leksawasdi N., Kasinrerk W., Tayapiwatana C., Butr-Indr B.
• Format: Article
• Language: English
• Published: Taylor and Francis Inc. 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-84906070208&partnerID=40&md5=4230ee3fd1ea8d418965500688d0fb1f; http://cmuir.cmu.ac.th/handle/6653943832/37558
• Institution: Chiang Mai University

The optimal culture condition for extracellular recombinant single chain variable fragment anti HIV-1 p17 protein (scFv anti-p17) production in Escherichia coli HB2151 was investigated by the sequential simplex optimization (SS) method. Five variable parameters were submitted in the fermentation pro...