Novel application of polioviral capsid: Development of a potent and prolonged oral calcitonin using polioviral binding ligand and Tat peptide

Context: Poor absorption and proteolytic degradation are major obstacles of orally administered peptide drugs including calcitonin. Cell penetrating peptides (CPPs) and receptor binding ligands are interesting tools for the application in the delivery of these drugs. Objective: To investigate the en...

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Main Authors: Manosroi J., Lohcharoenkal W., Gotz F., Werner R.G., Manosroi W., Manosroi A.
Format: Article
Language:English
Published: Informa Healthcare 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84905509678&partnerID=40&md5=b3e59450e66ee18186c32d4623f8ce58
http://cmuir.cmu.ac.th/handle/6653943832/37628
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-376282014-12-09T05:54:18Z Novel application of polioviral capsid: Development of a potent and prolonged oral calcitonin using polioviral binding ligand and Tat peptide Manosroi J. Lohcharoenkal W. Gotz F. Werner R.G. Manosroi W. Manosroi A. Context: Poor absorption and proteolytic degradation are major obstacles of orally administered peptide drugs including calcitonin. Cell penetrating peptides (CPPs) and receptor binding ligands are interesting tools for the application in the delivery of these drugs. Objective: To investigate the enhancements of in vitro and in vivo salmon calcitonin (sCT) activity by Tat, a trans-activating transcriptional peptide and VP1 peptide (V) from polioviral capsid. Materials and methods: Tat/sCT, V/sCT and V/Tat/sCT mixtures at various molar ratios were prepared and investigated for in vitro and in vivo activities of sCT. Results: Tat could increase in vitro sCT activity both in colon adenocarcinoma (HT-29) and mouth epidermal carcinoma (KB) cells. V/sCT (6:1) showed significant increase of intracellular calcium in HT-29 cells. V/Tat/sCT (6:1:1) gave highest increase of intracellular calcium in both cells. Oral administered Tat/sCT (1:1) showed comparable hypocalcemic effect to sCT injection with prolonged action. V/Tat/sCT (6:1:1) demonstrated hypocalcemic effect at 12 h after administration but no hypocalcemic effect was observed from V/sCT. Discussion: Positive charge from Tat might facilitate sCT uptake and absorption. Increasing of intracellular calcium in HT-29 cells by V but lacking of hypocalcemic effect from V/sCT in mice indicated the ligand-receptor mediated delivery of sCT by the interaction between V and PVR. Conclusion: Potential application of V and Tat in oral calcitonin delivery system was demonstrated. Further study in a proper PVR bearing host is still needed to provide more useful information for the application of V in the development of drug delivery systems. © 2014 Informa Healthcare USA, Inc. 2014-12-09T05:54:18Z 2014-12-09T05:54:18Z 2014 Article 03639045 10.3109/03639045.2013.809533 DDIPD http://www.scopus.com/inward/record.url?eid=2-s2.0-84905509678&partnerID=40&md5=b3e59450e66ee18186c32d4623f8ce58 http://cmuir.cmu.ac.th/handle/6653943832/37628 English Informa Healthcare
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Context: Poor absorption and proteolytic degradation are major obstacles of orally administered peptide drugs including calcitonin. Cell penetrating peptides (CPPs) and receptor binding ligands are interesting tools for the application in the delivery of these drugs. Objective: To investigate the enhancements of in vitro and in vivo salmon calcitonin (sCT) activity by Tat, a trans-activating transcriptional peptide and VP1 peptide (V) from polioviral capsid. Materials and methods: Tat/sCT, V/sCT and V/Tat/sCT mixtures at various molar ratios were prepared and investigated for in vitro and in vivo activities of sCT. Results: Tat could increase in vitro sCT activity both in colon adenocarcinoma (HT-29) and mouth epidermal carcinoma (KB) cells. V/sCT (6:1) showed significant increase of intracellular calcium in HT-29 cells. V/Tat/sCT (6:1:1) gave highest increase of intracellular calcium in both cells. Oral administered Tat/sCT (1:1) showed comparable hypocalcemic effect to sCT injection with prolonged action. V/Tat/sCT (6:1:1) demonstrated hypocalcemic effect at 12 h after administration but no hypocalcemic effect was observed from V/sCT. Discussion: Positive charge from Tat might facilitate sCT uptake and absorption. Increasing of intracellular calcium in HT-29 cells by V but lacking of hypocalcemic effect from V/sCT in mice indicated the ligand-receptor mediated delivery of sCT by the interaction between V and PVR. Conclusion: Potential application of V and Tat in oral calcitonin delivery system was demonstrated. Further study in a proper PVR bearing host is still needed to provide more useful information for the application of V in the development of drug delivery systems. © 2014 Informa Healthcare USA, Inc.
format Article
author Manosroi J.
Lohcharoenkal W.
Gotz F.
Werner R.G.
Manosroi W.
Manosroi A.
spellingShingle Manosroi J.
Lohcharoenkal W.
Gotz F.
Werner R.G.
Manosroi W.
Manosroi A.
Novel application of polioviral capsid: Development of a potent and prolonged oral calcitonin using polioviral binding ligand and Tat peptide
author_facet Manosroi J.
Lohcharoenkal W.
Gotz F.
Werner R.G.
Manosroi W.
Manosroi A.
author_sort Manosroi J.
title Novel application of polioviral capsid: Development of a potent and prolonged oral calcitonin using polioviral binding ligand and Tat peptide
title_short Novel application of polioviral capsid: Development of a potent and prolonged oral calcitonin using polioviral binding ligand and Tat peptide
title_full Novel application of polioviral capsid: Development of a potent and prolonged oral calcitonin using polioviral binding ligand and Tat peptide
title_fullStr Novel application of polioviral capsid: Development of a potent and prolonged oral calcitonin using polioviral binding ligand and Tat peptide
title_full_unstemmed Novel application of polioviral capsid: Development of a potent and prolonged oral calcitonin using polioviral binding ligand and Tat peptide
title_sort novel application of polioviral capsid: development of a potent and prolonged oral calcitonin using polioviral binding ligand and tat peptide
publisher Informa Healthcare
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84905509678&partnerID=40&md5=b3e59450e66ee18186c32d4623f8ce58
http://cmuir.cmu.ac.th/handle/6653943832/37628
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