Attenuation of oxidative damage and inflammatory responses by apigenin given to mice after irradiation

We determined the in vivo efficacy of apigenin, as an anti-oxidant and anti-inflammatory agent, given to mice after irradiation. Various concentrations of apigenin (0, 10, 20, and 40mg/kg body weight) were administered to mice by a single intraperitoneal injection 3hr after receiving 0 or 3Gy of 137...

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Main Authors: Rithidech,K.N., Tungjai,M., Reungpatthanaphong,P., Honikel,L.M., Simon,S.R.
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Published: Elsevier 2015
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spelling th-cmuir.6653943832-381072015-06-16T07:38:25Z Attenuation of oxidative damage and inflammatory responses by apigenin given to mice after irradiation Rithidech,K.N. Tungjai,M. Reungpatthanaphong,P. Honikel,L.M. Simon,S.R. Health, Toxicology and Mutagenesis Genetics We determined the in vivo efficacy of apigenin, as an anti-oxidant and anti-inflammatory agent, given to mice after irradiation. Various concentrations of apigenin (0, 10, 20, and 40mg/kg body weight) were administered to mice by a single intraperitoneal injection 3hr after receiving 0 or 3Gy of 137Cs gamma rays. Mice receiving vehicle only (no radiation and no apigenin) served as sham controls. We assessed the anti-oxidative activity of apigenin in vivo by measuring levels of 8-hydroxy-2-deoxy guanosine (8-OH-dG) in bone marrow (BM) cells, collected at days 3 and 10 after irradiation, from groups of mice (5 mice per treatment group) with or without apigenin treatment. Simultaneously, we evaluated the ability of apigenin to diminish radiation-induced inflammatory responses in bone-marrow-derived macrophages (BMDMs) from the same individual mice used for measuring the level of 8-OH-dG. To do this, the levels of activated nuclear factor-kappa B (NF-kappa B) and NF-kappa B-regulated pro-inflammatory cytokines [i.e. interleukin 1-beta (IL-1beta), IL-6, and tumor necrosis factor-alpha (TNF-alpha)] were measured in BMDMs. Our results indicated significant reductions (p<0.01 or <0.05) in the levels of 8-OH-dG in BM cells collected at both harvest times from irradiated mice receiving apigenin treatment, at all apigenin concentrations tested. Likewise, activation of NF-kappa B in BMDMs collected from gamma-irradiated mice that received apigenin was suppressed at both harvest times. Further, the levels of pro-inflammatory cytokines in gamma-irradiated mice treated with 20 or 40mg/kg body weight apigenin were significantly lower than those in mice receiving radiation only (p<0.01 or <0.05) even at day 10 post-irradiation. Additionally, the ratio of neutrophils to lymphocytes indicated that apigenin ameliorated radiation-induced hematological toxicity. Our study is the first to demonstrate the mitigative/therapeutic effects of apigenin given to mice after irradiation. © 2012 Elsevier B.V. 2015-06-16T07:38:25Z 2015-06-16T07:38:25Z 2012-12-12 Article 13835718 2-s2.0-84869111549 10.1016/j.mrgentox.2012.08.001 23010607 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84869111549&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38107 Elsevier
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Health, Toxicology and Mutagenesis
Genetics
spellingShingle Health, Toxicology and Mutagenesis
Genetics
Rithidech,K.N.
Tungjai,M.
Reungpatthanaphong,P.
Honikel,L.M.
Simon,S.R.
Attenuation of oxidative damage and inflammatory responses by apigenin given to mice after irradiation
description We determined the in vivo efficacy of apigenin, as an anti-oxidant and anti-inflammatory agent, given to mice after irradiation. Various concentrations of apigenin (0, 10, 20, and 40mg/kg body weight) were administered to mice by a single intraperitoneal injection 3hr after receiving 0 or 3Gy of 137Cs gamma rays. Mice receiving vehicle only (no radiation and no apigenin) served as sham controls. We assessed the anti-oxidative activity of apigenin in vivo by measuring levels of 8-hydroxy-2-deoxy guanosine (8-OH-dG) in bone marrow (BM) cells, collected at days 3 and 10 after irradiation, from groups of mice (5 mice per treatment group) with or without apigenin treatment. Simultaneously, we evaluated the ability of apigenin to diminish radiation-induced inflammatory responses in bone-marrow-derived macrophages (BMDMs) from the same individual mice used for measuring the level of 8-OH-dG. To do this, the levels of activated nuclear factor-kappa B (NF-kappa B) and NF-kappa B-regulated pro-inflammatory cytokines [i.e. interleukin 1-beta (IL-1beta), IL-6, and tumor necrosis factor-alpha (TNF-alpha)] were measured in BMDMs. Our results indicated significant reductions (p<0.01 or <0.05) in the levels of 8-OH-dG in BM cells collected at both harvest times from irradiated mice receiving apigenin treatment, at all apigenin concentrations tested. Likewise, activation of NF-kappa B in BMDMs collected from gamma-irradiated mice that received apigenin was suppressed at both harvest times. Further, the levels of pro-inflammatory cytokines in gamma-irradiated mice treated with 20 or 40mg/kg body weight apigenin were significantly lower than those in mice receiving radiation only (p<0.01 or <0.05) even at day 10 post-irradiation. Additionally, the ratio of neutrophils to lymphocytes indicated that apigenin ameliorated radiation-induced hematological toxicity. Our study is the first to demonstrate the mitigative/therapeutic effects of apigenin given to mice after irradiation. © 2012 Elsevier B.V.
format Article
author Rithidech,K.N.
Tungjai,M.
Reungpatthanaphong,P.
Honikel,L.M.
Simon,S.R.
author_facet Rithidech,K.N.
Tungjai,M.
Reungpatthanaphong,P.
Honikel,L.M.
Simon,S.R.
author_sort Rithidech,K.N.
title Attenuation of oxidative damage and inflammatory responses by apigenin given to mice after irradiation
title_short Attenuation of oxidative damage and inflammatory responses by apigenin given to mice after irradiation
title_full Attenuation of oxidative damage and inflammatory responses by apigenin given to mice after irradiation
title_fullStr Attenuation of oxidative damage and inflammatory responses by apigenin given to mice after irradiation
title_full_unstemmed Attenuation of oxidative damage and inflammatory responses by apigenin given to mice after irradiation
title_sort attenuation of oxidative damage and inflammatory responses by apigenin given to mice after irradiation
publisher Elsevier
publishDate 2015
url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84869111549&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/38107
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