Contribution of the serine kinase c-Jun N-terminal kinase (JNK) to oxidant-induced insulin resistance in isolated rat skeletal muscle

The specific and direct contribution of the stress-activated serine kinase c-Jun N-terminal kinase (JNK) in the development of oxidative stress-induced insulin resistance of the glucose transport system in mammalian skeletal muscle is not fully understood. We assessed the specific role of JNK in the...

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Main Authors: Santos,F.R., Diamond-Stanic,M.K., Prasannarong,M., Henriksen,E.J.
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Published: Informa Healthcare 2015
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http://cmuir.cmu.ac.th/handle/6653943832/38108
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spelling th-cmuir.6653943832-381082015-06-16T07:38:25Z Contribution of the serine kinase c-Jun N-terminal kinase (JNK) to oxidant-induced insulin resistance in isolated rat skeletal muscle Santos,F.R. Diamond-Stanic,M.K. Prasannarong,M. Henriksen,E.J. Physiology Physiology (medical) The specific and direct contribution of the stress-activated serine kinase c-Jun N-terminal kinase (JNK) in the development of oxidative stress-induced insulin resistance of the glucose transport system in mammalian skeletal muscle is not fully understood. We assessed the specific role of JNK in the development of insulin resistance caused by in vitro exposure of rat soleus muscle to low levels (30-40 μM) of the oxidant hydrogen peroxide (H2O2) for up to 6h. Oxidant exposure caused significant (p < 0.05) decreases in insulin-stimulated glucose transport activity (up to 42%) and Akt Ser 473 phosphorylation (up to 67%), and increased (up to 74%) phosphorylation (Thr183/Tyr185) of JNK1 and JNK2/3 isoforms. Importantly, insulin-stimulated glucose transport activity in the presence of H2O2 was moderately improved with the selective JNK inhibitor SP600125. These results indicate that activation of the serine kinase JNK contributes, at least in part, to oxidative stress-induced insulin resistance in isolated mammalian skeletal muscle. © 2012 Informa UK, Ltd. 2015-06-16T07:38:25Z 2015-06-16T07:38:25Z 2012-12-01 Article 13813455 2-s2.0-84869420795 10.3109/13813455.2012.713366 22916958 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84869420795&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38108 Informa Healthcare
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Physiology
Physiology (medical)
spellingShingle Physiology
Physiology (medical)
Santos,F.R.
Diamond-Stanic,M.K.
Prasannarong,M.
Henriksen,E.J.
Contribution of the serine kinase c-Jun N-terminal kinase (JNK) to oxidant-induced insulin resistance in isolated rat skeletal muscle
description The specific and direct contribution of the stress-activated serine kinase c-Jun N-terminal kinase (JNK) in the development of oxidative stress-induced insulin resistance of the glucose transport system in mammalian skeletal muscle is not fully understood. We assessed the specific role of JNK in the development of insulin resistance caused by in vitro exposure of rat soleus muscle to low levels (30-40 μM) of the oxidant hydrogen peroxide (H2O2) for up to 6h. Oxidant exposure caused significant (p < 0.05) decreases in insulin-stimulated glucose transport activity (up to 42%) and Akt Ser 473 phosphorylation (up to 67%), and increased (up to 74%) phosphorylation (Thr183/Tyr185) of JNK1 and JNK2/3 isoforms. Importantly, insulin-stimulated glucose transport activity in the presence of H2O2 was moderately improved with the selective JNK inhibitor SP600125. These results indicate that activation of the serine kinase JNK contributes, at least in part, to oxidative stress-induced insulin resistance in isolated mammalian skeletal muscle. © 2012 Informa UK, Ltd.
format Article
author Santos,F.R.
Diamond-Stanic,M.K.
Prasannarong,M.
Henriksen,E.J.
author_facet Santos,F.R.
Diamond-Stanic,M.K.
Prasannarong,M.
Henriksen,E.J.
author_sort Santos,F.R.
title Contribution of the serine kinase c-Jun N-terminal kinase (JNK) to oxidant-induced insulin resistance in isolated rat skeletal muscle
title_short Contribution of the serine kinase c-Jun N-terminal kinase (JNK) to oxidant-induced insulin resistance in isolated rat skeletal muscle
title_full Contribution of the serine kinase c-Jun N-terminal kinase (JNK) to oxidant-induced insulin resistance in isolated rat skeletal muscle
title_fullStr Contribution of the serine kinase c-Jun N-terminal kinase (JNK) to oxidant-induced insulin resistance in isolated rat skeletal muscle
title_full_unstemmed Contribution of the serine kinase c-Jun N-terminal kinase (JNK) to oxidant-induced insulin resistance in isolated rat skeletal muscle
title_sort contribution of the serine kinase c-jun n-terminal kinase (jnk) to oxidant-induced insulin resistance in isolated rat skeletal muscle
publisher Informa Healthcare
publishDate 2015
url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84869420795&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/38108
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