Enhanced production of functional extracellular single chain variable fragment against HIV-1 matrix protein from escherichia coli by sequential simplex optimization

• Main Authors: Intachai,K., Singboottra,P., Leksawasdi,N., Kasinrerk,W., Tayapiwatana,C., Butr-Indr,B.
• Format: Article
• Published: Taylor and Francis Ltd. 2015
• Subjects: Biochemistry; Biotechnology
• Online Access: http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84906070208&origin=inward; http://cmuir.cmu.ac.th/handle/6653943832/38122
• Institution: Chiang Mai University

The optimal culture condition for extracellular recombinant single chain variable fragment anti HIV-1 p17 protein (scFv anti-p17) production in Escherichia coli HB2151 was investigated by the sequential simplex optimization (SS) method. Five variable parameters were submitted in the fermentation pro...