Visualizing reactive oxygen species inside cancer cells after stimulation with polycyclic aromatic hydrocarbon via spontaneous formation of Au nanoclusters
© 2014 Elsevier B.V. All rights reserved. This research was designed to visualize cellular response - in particular the generation of intracellular ROS - to stimulation by polycyclic aromatic hydrocarbon (PAH). We used chloroauric acid (HAuCl4) as a molecular probe for visualizing intracellular ROS...
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| Main Authors: | , , |
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| Format: | Article |
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Elsevier
2015
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| Online Access: | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84912050707&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38129 |
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| Institution: | Chiang Mai University |
| Summary: | © 2014 Elsevier B.V. All rights reserved. This research was designed to visualize cellular response - in particular the generation of intracellular ROS - to stimulation by polycyclic aromatic hydrocarbon (PAH). We used chloroauric acid (HAuCl4) as a molecular probe for visualizing intracellular ROS generated by the stimulation of benzo[a]pyrene (BaP). The chloroauric acid undergoes a spontaneous reduction reaction, assisted by the intracellular ROS, into gold nanoclusters (AuNCs). As a result, we can visualize the ROS via optical imaging technique. According to MTT assay, the chloroauric acid exhibited good biocompatibility. The AuNCs produced in the cells were approximately 2-3 nm in diameter with a green fluorescent property. Cellular imaging showed that BaP induced the formation of AuNCs within the cells, leading to a high relative cellular fluorescent intensity with a considerable extent of scatter light. Thus, this probe is an efficient molecular imaging probe for visualizing intracellular ROS. |
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