β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity
© 2015 Elsevier GmbH. Na,K ATPase plays an important role in the regulation of Na<sup>+</sup> and K<sup>+</sup> ions that are required for normal resting membrane potential and various cellular functions. Na,K ATPase is composed of two subunits, α and β subunits. Engagement o...
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Urban und Fischer Verlag GmbH und Co. KG
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th-cmuir.6653943832-381392015-06-16T07:38:29Z β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity Chruewkamlow,N. Pata,S. Mahasongkram,K. Laopajon,W. Kasinrerk,W. Chiampanichayakul,S. Immunology and Allergy Immunology Hematology © 2015 Elsevier GmbH. Na,K ATPase plays an important role in the regulation of Na<sup>+</sup> and K<sup>+</sup> ions that are required for normal resting membrane potential and various cellular functions. Na,K ATPase is composed of two subunits, α and β subunits. Engagement of the β subunit by an agonistic monoclonal antibody (mAb) P-3E10 inhibited T cell activation and induced the G0/G1 cell cycle arrest. In addition, mAb P-3E10 decreased CD25 expression. The mAb P-3E10, however, did not inhibit the proliferation of cell lines and the phagocytosis activity of phagocytes, and did not interfere with the Na,K ATPase activity. These results indicate that mAb P-3E10 reacts to the β subunit and, as a consequence, brings about the regulation of the T cell activation without disturbing the Na,K pump activity. By sequential immunoprecipitation, we demonstrated the expression of the β3 subunit free form apart from the α subunit. In this study, we propose that the β3 subunits of Na,K ATPase are expressed separately from the α subunit, and play a role in regulation of the immune response. 2015-06-16T07:38:29Z 2015-06-16T07:38:29Z 2015-01-01 Article 01712985 2-s2.0-84926421480 10.1016/j.imbio.2014.11.017 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84926421480&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38139 Urban und Fischer Verlag GmbH und Co. KG |
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Immunology and Allergy Immunology Hematology Chruewkamlow,N. Pata,S. Mahasongkram,K. Laopajon,W. Kasinrerk,W. Chiampanichayakul,S. β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity |
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© 2015 Elsevier GmbH. Na,K ATPase plays an important role in the regulation of Na<sup>+</sup> and K<sup>+</sup> ions that are required for normal resting membrane potential and various cellular functions. Na,K ATPase is composed of two subunits, α and β subunits. Engagement of the β subunit by an agonistic monoclonal antibody (mAb) P-3E10 inhibited T cell activation and induced the G0/G1 cell cycle arrest. In addition, mAb P-3E10 decreased CD25 expression. The mAb P-3E10, however, did not inhibit the proliferation of cell lines and the phagocytosis activity of phagocytes, and did not interfere with the Na,K ATPase activity. These results indicate that mAb P-3E10 reacts to the β subunit and, as a consequence, brings about the regulation of the T cell activation without disturbing the Na,K pump activity. By sequential immunoprecipitation, we demonstrated the expression of the β3 subunit free form apart from the α subunit. In this study, we propose that the β3 subunits of Na,K ATPase are expressed separately from the α subunit, and play a role in regulation of the immune response. |
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Article |
author |
Chruewkamlow,N. Pata,S. Mahasongkram,K. Laopajon,W. Kasinrerk,W. Chiampanichayakul,S. |
author_facet |
Chruewkamlow,N. Pata,S. Mahasongkram,K. Laopajon,W. Kasinrerk,W. Chiampanichayakul,S. |
author_sort |
Chruewkamlow,N. |
title |
β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity |
title_short |
β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity |
title_full |
β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity |
title_fullStr |
β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity |
title_full_unstemmed |
β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity |
title_sort |
β3 subunit of na,k atpase regulates t cell activation with no involvement of na,k atpase activity |
publisher |
Urban und Fischer Verlag GmbH und Co. KG |
publishDate |
2015 |
url |
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84926421480&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38139 |
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