β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity

© 2015 Elsevier GmbH. Na,K ATPase plays an important role in the regulation of Na<sup>+</sup> and K<sup>+</sup> ions that are required for normal resting membrane potential and various cellular functions. Na,K ATPase is composed of two subunits, α and β subunits. Engagement o...

Full description

Saved in:
Bibliographic Details
Main Authors: Chruewkamlow,N., Pata,S., Mahasongkram,K., Laopajon,W., Kasinrerk,W., Chiampanichayakul,S.
Format: Article
Published: Urban und Fischer Verlag GmbH und Co. KG 2015
Subjects:
Online Access:http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84926421480&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/38139
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-38139
record_format dspace
spelling th-cmuir.6653943832-381392015-06-16T07:38:29Z β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity Chruewkamlow,N. Pata,S. Mahasongkram,K. Laopajon,W. Kasinrerk,W. Chiampanichayakul,S. Immunology and Allergy Immunology Hematology © 2015 Elsevier GmbH. Na,K ATPase plays an important role in the regulation of Na<sup>+</sup> and K<sup>+</sup> ions that are required for normal resting membrane potential and various cellular functions. Na,K ATPase is composed of two subunits, α and β subunits. Engagement of the β subunit by an agonistic monoclonal antibody (mAb) P-3E10 inhibited T cell activation and induced the G0/G1 cell cycle arrest. In addition, mAb P-3E10 decreased CD25 expression. The mAb P-3E10, however, did not inhibit the proliferation of cell lines and the phagocytosis activity of phagocytes, and did not interfere with the Na,K ATPase activity. These results indicate that mAb P-3E10 reacts to the β subunit and, as a consequence, brings about the regulation of the T cell activation without disturbing the Na,K pump activity. By sequential immunoprecipitation, we demonstrated the expression of the β3 subunit free form apart from the α subunit. In this study, we propose that the β3 subunits of Na,K ATPase are expressed separately from the α subunit, and play a role in regulation of the immune response. 2015-06-16T07:38:29Z 2015-06-16T07:38:29Z 2015-01-01 Article 01712985 2-s2.0-84926421480 10.1016/j.imbio.2014.11.017 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84926421480&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38139 Urban und Fischer Verlag GmbH und Co. KG
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Allergy
Immunology
Hematology
spellingShingle Immunology and Allergy
Immunology
Hematology
Chruewkamlow,N.
Pata,S.
Mahasongkram,K.
Laopajon,W.
Kasinrerk,W.
Chiampanichayakul,S.
β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity
description © 2015 Elsevier GmbH. Na,K ATPase plays an important role in the regulation of Na<sup>+</sup> and K<sup>+</sup> ions that are required for normal resting membrane potential and various cellular functions. Na,K ATPase is composed of two subunits, α and β subunits. Engagement of the β subunit by an agonistic monoclonal antibody (mAb) P-3E10 inhibited T cell activation and induced the G0/G1 cell cycle arrest. In addition, mAb P-3E10 decreased CD25 expression. The mAb P-3E10, however, did not inhibit the proliferation of cell lines and the phagocytosis activity of phagocytes, and did not interfere with the Na,K ATPase activity. These results indicate that mAb P-3E10 reacts to the β subunit and, as a consequence, brings about the regulation of the T cell activation without disturbing the Na,K pump activity. By sequential immunoprecipitation, we demonstrated the expression of the β3 subunit free form apart from the α subunit. In this study, we propose that the β3 subunits of Na,K ATPase are expressed separately from the α subunit, and play a role in regulation of the immune response.
format Article
author Chruewkamlow,N.
Pata,S.
Mahasongkram,K.
Laopajon,W.
Kasinrerk,W.
Chiampanichayakul,S.
author_facet Chruewkamlow,N.
Pata,S.
Mahasongkram,K.
Laopajon,W.
Kasinrerk,W.
Chiampanichayakul,S.
author_sort Chruewkamlow,N.
title β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity
title_short β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity
title_full β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity
title_fullStr β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity
title_full_unstemmed β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity
title_sort β3 subunit of na,k atpase regulates t cell activation with no involvement of na,k atpase activity
publisher Urban und Fischer Verlag GmbH und Co. KG
publishDate 2015
url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84926421480&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/38139
_version_ 1681421419043356672