Magnetic-EpCAM nanoprobe as a new platform for efficient targeting, isolating and imaging hepatocellular carcinoma
© The Royal Society of Chemistry 2015. Herein, magnetic-EpCAM nanoparticle (EpCAM-MNP) was developed and exploited as nanoprobe for targeting, isolating and imaging hepatocellular carcinoma. The nanoprobe was composed of two major components including aminosilane-coated iron oxide nanoparticles and...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Published: |
Royal Society of Chemistry
2015
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| Subjects: | |
| Online Access: | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84926633716&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38140 |
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| Institution: | Chiang Mai University |
| Summary: | © The Royal Society of Chemistry 2015. Herein, magnetic-EpCAM nanoparticle (EpCAM-MNP) was developed and exploited as nanoprobe for targeting, isolating and imaging hepatocellular carcinoma. The nanoprobe was composed of two major components including aminosilane-coated iron oxide nanoparticles and DNA-based EpCAM aptamers. Extensive studies were carried out to investigate its physico-chemical properties, magnetic relaxivity, as well as biocompatibility. The results indicated that the small size of the nanoprobe (HD < 100 nm) had high R2 relaxivity with good biocompatibility. Study on cellular accumulation and cellular uptake demonstrated high accumulation of EpCAM-MNP that was internalized via receptor-mediated endocytosis, as evidenced by TEM analysis. By using a magnetic-activated cell sorting (MACS) approach, the majority (∼96%) of the HepG2 cells were isolated, indicating the feasibility of our nanoprobe for isolating EpCAM-positive cells in clinical application. Because of the high intracellular accumulation and the high R2 relaxivity of EpCAM-MNP, it can be used for quantitative MRI detection of cancer cells with high sensitivity. |
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