Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study
© 2015 Elsevier Inc. All rights reserved. α<sup>0</sup>-Thalassemia occurs from a deletion of 2 linked α-globin genes and interaction of these defective genes leads to hemoglobin (Hb) Bart's hydrops fetalis, the most severe and lethal thalassemia syndrome. Identification of α<sup...
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th-cmuir.6653943832-381452015-06-16T07:38:30Z Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study Winichagoon P. Kumpan P. Holmes P. Finlayson J. Newbound C. Kabral A. Li B. Nuinoon M. Fawcett T. Tayapiwatana C. Kasinrerk W. Fucharoen S. Medicine (all) Biochemistry (medical) Public Health, Environmental and Occupational Health © 2015 Elsevier Inc. All rights reserved. α<sup>0</sup>-Thalassemia occurs from a deletion of 2 linked α-globin genes and interaction of these defective genes leads to hemoglobin (Hb) Bart's hydrops fetalis, the most severe and lethal thalassemia syndrome. Identification of α<sup>0</sup>-thalassemia carriers is thus essential for the prevention and control program. An immunochromatographic (IC) strip test was developed for rapid screening of α<sup>0</sup>-thalassemia by testing for Hb Bart's in the blood samples using a specific monoclonal antibody against Hb Bart's. To evaluate its sensitivity and specificity, the IC strip test was assessed in a cohort with various thalassemia genotypes from 4 different laboratories in Thailand and Australia. The result showed 97% sensitivity in α-thalassemia carriers with 2 α-globin genes deletion and Hb H disease. This is, in particular, the useful rapid screening test for regions where β-thalassemia and homozygous Hb E are also common. Similar hematologic and Hb data make it impossible to address the concomitant inheritance of α<sup>0</sup>-thalassemia in these samples without polymerase chain reaction (PCR)-based techniques, leading to misdiagnosis of the risk of having Hb Bart's hydrops fetalis. However, α-globin genotyping should be carried out in samples with positive IC strip as positive reactivity was also observed in homozygous α<sup>+</sup>-thalassemia carriers who have 2 trans α-globin gene deletions. These results indicate that in combination with red blood cell indices, the IC strip test could rule out mass populations for further α<sup>0</sup>-thalassemia detection by PCR-based analysis. The Alpha Thal IC strip also has the potential to replace testing for Hb H inclusion bodies, as it appears to be more sensitive, specific, and less labor intensive. 2015-06-16T07:38:30Z 2015-06-16T07:38:30Z 2015-01-01 Article 19315244 2-s2.0-84929191929 10.1016/j.trsl.2014.10.013 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84929191929&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38145 Mosby Inc. |
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Medicine (all) Biochemistry (medical) Public Health, Environmental and Occupational Health Winichagoon P. Kumpan P. Holmes P. Finlayson J. Newbound C. Kabral A. Li B. Nuinoon M. Fawcett T. Tayapiwatana C. Kasinrerk W. Fucharoen S. Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study |
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© 2015 Elsevier Inc. All rights reserved. α<sup>0</sup>-Thalassemia occurs from a deletion of 2 linked α-globin genes and interaction of these defective genes leads to hemoglobin (Hb) Bart's hydrops fetalis, the most severe and lethal thalassemia syndrome. Identification of α<sup>0</sup>-thalassemia carriers is thus essential for the prevention and control program. An immunochromatographic (IC) strip test was developed for rapid screening of α<sup>0</sup>-thalassemia by testing for Hb Bart's in the blood samples using a specific monoclonal antibody against Hb Bart's. To evaluate its sensitivity and specificity, the IC strip test was assessed in a cohort with various thalassemia genotypes from 4 different laboratories in Thailand and Australia. The result showed 97% sensitivity in α-thalassemia carriers with 2 α-globin genes deletion and Hb H disease. This is, in particular, the useful rapid screening test for regions where β-thalassemia and homozygous Hb E are also common. Similar hematologic and Hb data make it impossible to address the concomitant inheritance of α<sup>0</sup>-thalassemia in these samples without polymerase chain reaction (PCR)-based techniques, leading to misdiagnosis of the risk of having Hb Bart's hydrops fetalis. However, α-globin genotyping should be carried out in samples with positive IC strip as positive reactivity was also observed in homozygous α<sup>+</sup>-thalassemia carriers who have 2 trans α-globin gene deletions. These results indicate that in combination with red blood cell indices, the IC strip test could rule out mass populations for further α<sup>0</sup>-thalassemia detection by PCR-based analysis. The Alpha Thal IC strip also has the potential to replace testing for Hb H inclusion bodies, as it appears to be more sensitive, specific, and less labor intensive. |
format |
Article |
author |
Winichagoon P. Kumpan P. Holmes P. Finlayson J. Newbound C. Kabral A. Li B. Nuinoon M. Fawcett T. Tayapiwatana C. Kasinrerk W. Fucharoen S. |
author_facet |
Winichagoon P. Kumpan P. Holmes P. Finlayson J. Newbound C. Kabral A. Li B. Nuinoon M. Fawcett T. Tayapiwatana C. Kasinrerk W. Fucharoen S. |
author_sort |
Winichagoon P. |
title |
Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study |
title_short |
Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study |
title_full |
Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study |
title_fullStr |
Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study |
title_full_unstemmed |
Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study |
title_sort |
validation of the immunochromatographic strip for α-thalassemia screening: a multicenter study |
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Mosby Inc. |
publishDate |
2015 |
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http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84929191929&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38145 |
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1681421420155895808 |