Characterization of an MPLP6, a gene coding for a yeast phase specific, antigenic mannoprotein in Penicillium marneffei

A gene encoding an antigenic mannoprotein of Penicillium marneffei, MPLP6, was isolated by an antibody screening approach and characterized. The polypeptide chain containing deduced 220 amino acids has a predicted molecular mass of 24 kDa. It has high similarity to Mp1p, the first mannoprotein antig...

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Main Authors: Pongpom,M., Vanittanakom,N.
Format: Article
Published: Informa Healthcare 2015
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Online Access:http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=78650353472&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/38179
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-381792015-06-16T07:46:30Z Characterization of an MPLP6, a gene coding for a yeast phase specific, antigenic mannoprotein in Penicillium marneffei Pongpom,M. Vanittanakom,N. Veterinary (all) Infectious Diseases A gene encoding an antigenic mannoprotein of Penicillium marneffei, MPLP6, was isolated by an antibody screening approach and characterized. The polypeptide chain containing deduced 220 amino acids has a predicted molecular mass of 24 kDa. It has high similarity to Mp1p, the first mannoprotein antigen isolated from P. marneffei. The polypeptide sequence presents the property of cell wall mannoproteins by containing a putative N-terminal signal peptide and potential O-linked glycosylation sites. However, absence of a GPI-anchored signal sequence suggested that this protein is secreted. The MPLP6 transcript was present specifically in the pathogenic yeast form. The transcript was completely absent in the mold phase and conidia. The fusion protein produced in E. coli was Western immunoblotted with P. marneffei-infected human sera and 95% of the patients' sera were positive in the assay. None of the sera obtained from patients with aspergillosis, tuberculosis, histoplasmosis or cryptococcosis tested positive. These results suggest that Mplp6 can be used as a marker in a serodiagnostic assay. © 2011 ISHAM. 2015-06-16T07:46:30Z 2015-06-16T07:46:30Z 2010-01-01 Article 13693786 2-s2.0-78650353472 10.3109/13693786.2010.496796 20608782 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=78650353472&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38179 Informa Healthcare
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Veterinary (all)
Infectious Diseases
spellingShingle Veterinary (all)
Infectious Diseases
Pongpom,M.
Vanittanakom,N.
Characterization of an MPLP6, a gene coding for a yeast phase specific, antigenic mannoprotein in Penicillium marneffei
description A gene encoding an antigenic mannoprotein of Penicillium marneffei, MPLP6, was isolated by an antibody screening approach and characterized. The polypeptide chain containing deduced 220 amino acids has a predicted molecular mass of 24 kDa. It has high similarity to Mp1p, the first mannoprotein antigen isolated from P. marneffei. The polypeptide sequence presents the property of cell wall mannoproteins by containing a putative N-terminal signal peptide and potential O-linked glycosylation sites. However, absence of a GPI-anchored signal sequence suggested that this protein is secreted. The MPLP6 transcript was present specifically in the pathogenic yeast form. The transcript was completely absent in the mold phase and conidia. The fusion protein produced in E. coli was Western immunoblotted with P. marneffei-infected human sera and 95% of the patients' sera were positive in the assay. None of the sera obtained from patients with aspergillosis, tuberculosis, histoplasmosis or cryptococcosis tested positive. These results suggest that Mplp6 can be used as a marker in a serodiagnostic assay. © 2011 ISHAM.
format Article
author Pongpom,M.
Vanittanakom,N.
author_facet Pongpom,M.
Vanittanakom,N.
author_sort Pongpom,M.
title Characterization of an MPLP6, a gene coding for a yeast phase specific, antigenic mannoprotein in Penicillium marneffei
title_short Characterization of an MPLP6, a gene coding for a yeast phase specific, antigenic mannoprotein in Penicillium marneffei
title_full Characterization of an MPLP6, a gene coding for a yeast phase specific, antigenic mannoprotein in Penicillium marneffei
title_fullStr Characterization of an MPLP6, a gene coding for a yeast phase specific, antigenic mannoprotein in Penicillium marneffei
title_full_unstemmed Characterization of an MPLP6, a gene coding for a yeast phase specific, antigenic mannoprotein in Penicillium marneffei
title_sort characterization of an mplp6, a gene coding for a yeast phase specific, antigenic mannoprotein in penicillium marneffei
publisher Informa Healthcare
publishDate 2015
url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=78650353472&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/38179
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