Houttuynia cordata thunb fraction induces human leukemic molt-4 cell apoptosis through the endoplasmic reticulum stress pathway

Houttuynia cordata Thunb (HCT) is a native herb found in Southeast Asia which features various pharmacological activities against allergy, inflammation, viral and bacterial infection, and cancer. The aims of this study were to determine the cytotoxic effect of 6 fractions obtained from silica gel co...

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Main Authors: Prommaban,A., Kodchakorn,K., Kongtawelert,P., Banjerdpongchai,R.
Format: Article
Published: Asian Pacific Organization for Cancer Prevention 2015
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http://cmuir.cmu.ac.th/handle/6653943832/38250
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-382502015-06-16T07:46:44Z Houttuynia cordata thunb fraction induces human leukemic molt-4 cell apoptosis through the endoplasmic reticulum stress pathway Prommaban,A. Kodchakorn,K. Kongtawelert,P. Banjerdpongchai,R. Cancer Research Oncology Epidemiology Public Health, Environmental and Occupational Health Houttuynia cordata Thunb (HCT) is a native herb found in Southeast Asia which features various pharmacological activities against allergy, inflammation, viral and bacterial infection, and cancer. The aims of this study were to determine the cytotoxic effect of 6 fractions obtained from silica gel column chromatography of alcoholic HCT extract on human leukemic Molt-4 cells and demonstrate mechanisms of cell death. Six HCT fractions were cytotoxic to human lymphoblastic leukemic Molt-4 cells in a dose-dependent manner by MTT assay, fraction 4 exerting the greatest effects. Treatment with IC50 of HCT fraction 4 significantly induced Molt-4 apoptosis detected by annexinV-FITC/propidium iodide for externalization of phosphatidylserine to the outer layer of cell membrane. The mitochondrial transmembrane potential was reduced in HCT fraction 4-treated Molt-4 cells. Moreover, decreased expression of Bcl-xl and increased levels of Smac/Diablo, Bax and GRP78 proteins were noted on immunoblotting. In conclusion, HCT fraction 4 induces Molt-4 apoptosis cell through an endoplasmic reticulum stress pathway. 2015-06-16T07:46:44Z 2015-06-16T07:46:44Z 2012-12-01 Article 15137368 2-s2.0-84872678720 10.7314/APJCP.2012.13.5.1977 22901157 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84872678720&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38250 Asian Pacific Organization for Cancer Prevention
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Cancer Research
Oncology
Epidemiology
Public Health, Environmental and Occupational Health
spellingShingle Cancer Research
Oncology
Epidemiology
Public Health, Environmental and Occupational Health
Prommaban,A.
Kodchakorn,K.
Kongtawelert,P.
Banjerdpongchai,R.
Houttuynia cordata thunb fraction induces human leukemic molt-4 cell apoptosis through the endoplasmic reticulum stress pathway
description Houttuynia cordata Thunb (HCT) is a native herb found in Southeast Asia which features various pharmacological activities against allergy, inflammation, viral and bacterial infection, and cancer. The aims of this study were to determine the cytotoxic effect of 6 fractions obtained from silica gel column chromatography of alcoholic HCT extract on human leukemic Molt-4 cells and demonstrate mechanisms of cell death. Six HCT fractions were cytotoxic to human lymphoblastic leukemic Molt-4 cells in a dose-dependent manner by MTT assay, fraction 4 exerting the greatest effects. Treatment with IC50 of HCT fraction 4 significantly induced Molt-4 apoptosis detected by annexinV-FITC/propidium iodide for externalization of phosphatidylserine to the outer layer of cell membrane. The mitochondrial transmembrane potential was reduced in HCT fraction 4-treated Molt-4 cells. Moreover, decreased expression of Bcl-xl and increased levels of Smac/Diablo, Bax and GRP78 proteins were noted on immunoblotting. In conclusion, HCT fraction 4 induces Molt-4 apoptosis cell through an endoplasmic reticulum stress pathway.
format Article
author Prommaban,A.
Kodchakorn,K.
Kongtawelert,P.
Banjerdpongchai,R.
author_facet Prommaban,A.
Kodchakorn,K.
Kongtawelert,P.
Banjerdpongchai,R.
author_sort Prommaban,A.
title Houttuynia cordata thunb fraction induces human leukemic molt-4 cell apoptosis through the endoplasmic reticulum stress pathway
title_short Houttuynia cordata thunb fraction induces human leukemic molt-4 cell apoptosis through the endoplasmic reticulum stress pathway
title_full Houttuynia cordata thunb fraction induces human leukemic molt-4 cell apoptosis through the endoplasmic reticulum stress pathway
title_fullStr Houttuynia cordata thunb fraction induces human leukemic molt-4 cell apoptosis through the endoplasmic reticulum stress pathway
title_full_unstemmed Houttuynia cordata thunb fraction induces human leukemic molt-4 cell apoptosis through the endoplasmic reticulum stress pathway
title_sort houttuynia cordata thunb fraction induces human leukemic molt-4 cell apoptosis through the endoplasmic reticulum stress pathway
publisher Asian Pacific Organization for Cancer Prevention
publishDate 2015
url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84872678720&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/38250
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