Optimization, characterization and stability of essential oils blend loaded nanoemulsions by PIC technique for anti-tyrosinase activity

• Main Authors: Nantarat T., Chansakaow S., Leelapornpisid P.
• Format: Article
• Published: IJPPS 2015
• Subjects: Pharmaceutical Science; Pharmacology
• Online Access: http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84924419536&origin=inward; http://cmuir.cmu.ac.th/handle/6653943832/38560
• Institution: Chiang Mai University

© 2015, International Journal of Pharmacy and Pharmaceutical Science. All rights reserved. Objective: This study was proposed to develop nanoemulsions loading essential oils blend with anti-tyrosinase activity prepared by phase inversion composition technique (PIC). Methods: The nanoemulsions were formulated using the essential oils blend (EB) mixed with virgin coconut oil as carrier oil. PEG 40 hydrogenated castor oil and sorbitan monooleate were used as surfactant system. The effect of surfactant-to-oil ratio (SOR) and surfactant mixture concentration were determined. The EB loaded nanoemulsions were then characterized for their physical appearances, droplet size, zeta potential and mushroom tyrosinase inhibitory activity. Moreover, the stability under various storage conditions was also determined. Results: The results revealed that all the produced nanoemulsions were highly stable under various storage conditions with an average droplet size between 29.55 to 37.12 nm. The polydispersity index (PDI) values of all formulas were less than 0.2 and their zeta potentials ranged between -14.51 to -20.40 mV. Additionally, the EB loaded nanoemulsions presented good inhibitory effect on mushroom tyrosinase activity. Conclusion: The loading of the essential oils blend into nanoemulsions could be successfully prepared by phase inversion composition technique (PIC) that improved their stability and decreased the volatilization of the loaded essential oils.