Influence of plantlet age and different soilless culture on acclimatization of stemona curtisii Hook.f

The aim of this experiment was to study the optimal age of Stemona curtisii plantlets for acclimatization. The in vitro shoots of S. curtisii were cultured on Murashige and Skoog solid medium supplemented with 1 mg L-1 naphthalene acetic acid to induce roots. Then, the plantlets from in vitro cultur...

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Main Authors: Palee J., Dheeranupattana S., Jatisatienr A., Wangkarn S., Mungkornasawakul P., Pyne S., Ung A., Sastraruji T.
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Published: Asian Network for Scientific Information 2015
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http://cmuir.cmu.ac.th/handle/6653943832/38663
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-386632015-06-16T07:53:51Z Influence of plantlet age and different soilless culture on acclimatization of stemona curtisii Hook.f Palee J. Dheeranupattana S. Jatisatienr A. Wangkarn S. Mungkornasawakul P. Pyne S. Ung A. Sastraruji T. Plant Science Agronomy and Crop Science The aim of this experiment was to study the optimal age of Stemona curtisii plantlets for acclimatization. The in vitro shoots of S. curtisii were cultured on Murashige and Skoog solid medium supplemented with 1 mg L-1 naphthalene acetic acid to induce roots. Then, the plantlets from in vitro culture of 4, 8 and 12 week-old were transferred into the soil and their survival rate during the acclimatization process in the greenhouse was investigated. It was found that the 8 week-old plantlets had the highest survival rate of 80%. However, to enhance the survival rate of these plantlets, a soilless culture technique as a possible approach for the acclimatization was considered. Eight week-old plantlets were transferred into either, soil, a hydroponic system (nutrient film technique), coconut fiber or sand. The plantlets which were cultivated in coconut fiber or hydroponic culture showed 100% survival rate with the highest average number of new roots per plant. However, the mean root length of the plantlets grown in hydroponic system was significantly higher than that grown in coconut fiber. © 2012 Asian Network for Scientific Information. 2015-06-16T07:53:50Z 2015-06-16T07:53:50Z 2012-12-01 Article 16823974 2-s2.0-84871854300 10.3923/ajps.2012.294.299 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84871854300&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38663 Asian Network for Scientific Information
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Plant Science
Agronomy and Crop Science
spellingShingle Plant Science
Agronomy and Crop Science
Palee J.
Dheeranupattana S.
Jatisatienr A.
Wangkarn S.
Mungkornasawakul P.
Pyne S.
Ung A.
Sastraruji T.
Influence of plantlet age and different soilless culture on acclimatization of stemona curtisii Hook.f
description The aim of this experiment was to study the optimal age of Stemona curtisii plantlets for acclimatization. The in vitro shoots of S. curtisii were cultured on Murashige and Skoog solid medium supplemented with 1 mg L-1 naphthalene acetic acid to induce roots. Then, the plantlets from in vitro culture of 4, 8 and 12 week-old were transferred into the soil and their survival rate during the acclimatization process in the greenhouse was investigated. It was found that the 8 week-old plantlets had the highest survival rate of 80%. However, to enhance the survival rate of these plantlets, a soilless culture technique as a possible approach for the acclimatization was considered. Eight week-old plantlets were transferred into either, soil, a hydroponic system (nutrient film technique), coconut fiber or sand. The plantlets which were cultivated in coconut fiber or hydroponic culture showed 100% survival rate with the highest average number of new roots per plant. However, the mean root length of the plantlets grown in hydroponic system was significantly higher than that grown in coconut fiber. © 2012 Asian Network for Scientific Information.
format Article
author Palee J.
Dheeranupattana S.
Jatisatienr A.
Wangkarn S.
Mungkornasawakul P.
Pyne S.
Ung A.
Sastraruji T.
author_facet Palee J.
Dheeranupattana S.
Jatisatienr A.
Wangkarn S.
Mungkornasawakul P.
Pyne S.
Ung A.
Sastraruji T.
author_sort Palee J.
title Influence of plantlet age and different soilless culture on acclimatization of stemona curtisii Hook.f
title_short Influence of plantlet age and different soilless culture on acclimatization of stemona curtisii Hook.f
title_full Influence of plantlet age and different soilless culture on acclimatization of stemona curtisii Hook.f
title_fullStr Influence of plantlet age and different soilless culture on acclimatization of stemona curtisii Hook.f
title_full_unstemmed Influence of plantlet age and different soilless culture on acclimatization of stemona curtisii Hook.f
title_sort influence of plantlet age and different soilless culture on acclimatization of stemona curtisii hook.f
publisher Asian Network for Scientific Information
publishDate 2015
url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84871854300&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/38663
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