Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (Chlorophyta) using ISSR and rbcL markers

© 2014. Spirogyra is found in a wide range of habitats, including small stagnant water bodies, rivers, and streams. Spirogyra ellipsospora is common in northern Thailand. Species identification of the Spirogyra species based only on morphological characteristics can be difficult. A reliable and accu...

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Main Authors: Wongsawad,P., Peerapornpisal,Y.
Format: Article
Published: King Saud University 2015
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http://cmuir.cmu.ac.th/handle/6653943832/38781
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-387812015-06-16T07:54:13Z Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (Chlorophyta) using ISSR and rbcL markers Wongsawad,P. Peerapornpisal,Y. Agricultural and Biological Sciences (all) © 2014. Spirogyra is found in a wide range of habitats, including small stagnant water bodies, rivers, and streams. Spirogyra ellipsospora is common in northern Thailand. Species identification of the Spirogyra species based only on morphological characteristics can be difficult. A reliable and accurate method is required to evaluate genetic variations. This study aims to apply molecular approaches for the identification of S. ellipsospora using microsatellites and rbcL markers. Based on DNA sequencing, the rbcL gene was sequenced and the data was analyzed using the BLAST (Basic Local Alignment Search Tool) program in the NCBI (National Center for Biotechnology Information) database. The sequence of S. ellipsospora from this study revealed definitive identity matches in the range of 99% for the consensus sequences of S. ellipsospora. The 10 primers of ISSR could be amplified by 92 amplification fragments. The DNA fragments and the rbcL sequence data grouped the Spirogyra specimens into two distinct clusters. 2015-06-16T07:54:13Z 2015-06-16T07:54:13Z 2014-01-01 Article 1319562X 2-s2.0-84908094057 10.1016/j.sjbs.2014.01.003 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84908094057&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38781 King Saud University
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences (all)
spellingShingle Agricultural and Biological Sciences (all)
Wongsawad,P.
Peerapornpisal,Y.
Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (Chlorophyta) using ISSR and rbcL markers
description © 2014. Spirogyra is found in a wide range of habitats, including small stagnant water bodies, rivers, and streams. Spirogyra ellipsospora is common in northern Thailand. Species identification of the Spirogyra species based only on morphological characteristics can be difficult. A reliable and accurate method is required to evaluate genetic variations. This study aims to apply molecular approaches for the identification of S. ellipsospora using microsatellites and rbcL markers. Based on DNA sequencing, the rbcL gene was sequenced and the data was analyzed using the BLAST (Basic Local Alignment Search Tool) program in the NCBI (National Center for Biotechnology Information) database. The sequence of S. ellipsospora from this study revealed definitive identity matches in the range of 99% for the consensus sequences of S. ellipsospora. The 10 primers of ISSR could be amplified by 92 amplification fragments. The DNA fragments and the rbcL sequence data grouped the Spirogyra specimens into two distinct clusters.
format Article
author Wongsawad,P.
Peerapornpisal,Y.
author_facet Wongsawad,P.
Peerapornpisal,Y.
author_sort Wongsawad,P.
title Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (Chlorophyta) using ISSR and rbcL markers
title_short Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (Chlorophyta) using ISSR and rbcL markers
title_full Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (Chlorophyta) using ISSR and rbcL markers
title_fullStr Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (Chlorophyta) using ISSR and rbcL markers
title_full_unstemmed Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (Chlorophyta) using ISSR and rbcL markers
title_sort molecular identification and phylogenetic relationship of green algae, spirogyra ellipsospora (chlorophyta) using issr and rbcl markers
publisher King Saud University
publishDate 2015
url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84908094057&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/38781
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