Inhibitory effect of mammea E/BB from Mammea siamensis seed extract on Wilms' tumour 1 protein expression in a K562 leukaemic cell line

© 2015 Taylor & Francis Mammea siamensis is used in traditional Thai medicine. This study was designed to extract and isolate an active compound from the M. siamensis seeds and to investigate its activity on Wilms' tumour 1 (WT1) protein expression in K562 cells. WT1 is a transcription fact...

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Main Authors: Rungrojsakul,M., Saiai,A., Ampasavate,C., Anuchapreeda,S., Okonogi,S.
Format: Article
Published: Taylor and Francis Ltd. 2015
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http://cmuir.cmu.ac.th/handle/6653943832/38900
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-389002015-06-16T07:54:32Z Inhibitory effect of mammea E/BB from Mammea siamensis seed extract on Wilms' tumour 1 protein expression in a K562 leukaemic cell line Rungrojsakul,M. Saiai,A. Ampasavate,C. Anuchapreeda,S. Okonogi,S. Plant Science Analytical Chemistry Organic Chemistry Biochemistry © 2015 Taylor & Francis Mammea siamensis is used in traditional Thai medicine. This study was designed to extract and isolate an active compound from the M. siamensis seeds and to investigate its activity on Wilms' tumour 1 (WT1) protein expression in K562 cells. WT1 is a transcription factor that stimulates cell proliferation. The ethanol saraphi seed (ESS) extract was fractionated using n-hexane, ethyl acetate, n-butanol and water to obtain n-hexane saraphi seed (HSS), ethyl acetate saraphi seed (EASS), n-butanol saraphi seed (BSS), and water saraphi seed (WSS) extracts, respectively. The ESS, HSS and EASS extracts had strong cytotoxic effects on K562 cells in the MTT assay. All three fractions decreased WT1 protein levels and decreased total cell numbers. The HSS extract decreased the WT1 protein levels in a time- and dose-dependent manner. HPLC and NMR analyses indicated that the active compound of HSS was mammea E/BB. M. siamensis seeds are thus identified as a promising source of bioactive compounds for potential inhibition of WT1 protein expression. 2015-06-16T07:54:32Z 2015-06-16T07:54:32Z 2015-03-04 Article in Press 14786419 2-s2.0-84924149308 10.1080/14786419.2015.1017491 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84924149308&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38900 Taylor and Francis Ltd.
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Plant Science
Analytical Chemistry
Organic Chemistry
Biochemistry
spellingShingle Plant Science
Analytical Chemistry
Organic Chemistry
Biochemistry
Rungrojsakul,M.
Saiai,A.
Ampasavate,C.
Anuchapreeda,S.
Okonogi,S.
Inhibitory effect of mammea E/BB from Mammea siamensis seed extract on Wilms' tumour 1 protein expression in a K562 leukaemic cell line
description © 2015 Taylor & Francis Mammea siamensis is used in traditional Thai medicine. This study was designed to extract and isolate an active compound from the M. siamensis seeds and to investigate its activity on Wilms' tumour 1 (WT1) protein expression in K562 cells. WT1 is a transcription factor that stimulates cell proliferation. The ethanol saraphi seed (ESS) extract was fractionated using n-hexane, ethyl acetate, n-butanol and water to obtain n-hexane saraphi seed (HSS), ethyl acetate saraphi seed (EASS), n-butanol saraphi seed (BSS), and water saraphi seed (WSS) extracts, respectively. The ESS, HSS and EASS extracts had strong cytotoxic effects on K562 cells in the MTT assay. All three fractions decreased WT1 protein levels and decreased total cell numbers. The HSS extract decreased the WT1 protein levels in a time- and dose-dependent manner. HPLC and NMR analyses indicated that the active compound of HSS was mammea E/BB. M. siamensis seeds are thus identified as a promising source of bioactive compounds for potential inhibition of WT1 protein expression.
format Article
author Rungrojsakul,M.
Saiai,A.
Ampasavate,C.
Anuchapreeda,S.
Okonogi,S.
author_facet Rungrojsakul,M.
Saiai,A.
Ampasavate,C.
Anuchapreeda,S.
Okonogi,S.
author_sort Rungrojsakul,M.
title Inhibitory effect of mammea E/BB from Mammea siamensis seed extract on Wilms' tumour 1 protein expression in a K562 leukaemic cell line
title_short Inhibitory effect of mammea E/BB from Mammea siamensis seed extract on Wilms' tumour 1 protein expression in a K562 leukaemic cell line
title_full Inhibitory effect of mammea E/BB from Mammea siamensis seed extract on Wilms' tumour 1 protein expression in a K562 leukaemic cell line
title_fullStr Inhibitory effect of mammea E/BB from Mammea siamensis seed extract on Wilms' tumour 1 protein expression in a K562 leukaemic cell line
title_full_unstemmed Inhibitory effect of mammea E/BB from Mammea siamensis seed extract on Wilms' tumour 1 protein expression in a K562 leukaemic cell line
title_sort inhibitory effect of mammea e/bb from mammea siamensis seed extract on wilms' tumour 1 protein expression in a k562 leukaemic cell line
publisher Taylor and Francis Ltd.
publishDate 2015
url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84924149308&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/38900
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