Diversity of actinobacteria associated with Nostoc commune Vaucher ex Bornet & Flahault macrocolonies

© 2015 Springer-Verlag Berlin Heidelberg and the University of Milan Nostoc commune is a widely distributed freshwater cyanobacterium which grows on moist soil in Thailand, forming large or massive macroscopic colonies, called thallus, that are consumed as food by local people in the northeast regio...

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Main Authors: Inthasotti,T., Pathom-Aree,W.
Format: Article
Published: Springer Heidelberg 2015
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spelling th-cmuir.6653943832-389022015-06-16T07:54:32Z Diversity of actinobacteria associated with Nostoc commune Vaucher ex Bornet & Flahault macrocolonies Inthasotti,T. Pathom-Aree,W. Applied Microbiology and Biotechnology © 2015 Springer-Verlag Berlin Heidelberg and the University of Milan Nostoc commune is a widely distributed freshwater cyanobacterium which grows on moist soil in Thailand, forming large or massive macroscopic colonies, called thallus, that are consumed as food by local people in the northeast region of Thailand. We have studied the diversity of actinobacteria associated with macroscopic thalli of N. commune using a 16S rRNA gene clone library. A total of 70 clones were sequenced, and 45 clones were found to cluster in ten actinobacterial families, namely, Microbacteriaceae, Dermacoccaceae, Pseudonocardiaceae, Intrasporangiaceae, Micrococcaceae, Solirubrobacteraceae, Corynebacteriaceae, Actinomycetaceae, Mycobacteriaceae, and Micromonosporaceae. The majority of the clones were closely related to Arthrobacter oryzae (5 clones, 11.1 %), Micrococcus endophyticus (4 clones, 8.9 %), and Pseudonocardia ailaonensis (4 clones, 8.9 %). Actinobacterial diversity was high, with a Shannon–Wiener index of 3.25 and a Chao1 index of 187.0 ribotypes with 71.11 % coverage. A total of 57 strains were isolated using 12 different media, and 43 isolates were selected for phylogenetic analysis on the basis of their 16S rRNA gene sequences. The results showed that these isolates belonged to six known genera, including Actinomadura, Arthrobacter, Gordonia, Rhodococcus, Sanguibacter, and Streptomyces. These results suggest that a diverse actinobacterial community is associated with macroscopic thalli of N. commune and that potential novel taxa may exist within this environment. 2015-06-16T07:54:32Z 2015-06-16T07:54:32Z 2015-03-10 Article in Press 15904261 2-s2.0-84924351896 10.1007/s13213-015-1063-8 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84924351896&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38902 Springer Heidelberg
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Applied Microbiology and Biotechnology
spellingShingle Applied Microbiology and Biotechnology
Inthasotti,T.
Pathom-Aree,W.
Diversity of actinobacteria associated with Nostoc commune Vaucher ex Bornet & Flahault macrocolonies
description © 2015 Springer-Verlag Berlin Heidelberg and the University of Milan Nostoc commune is a widely distributed freshwater cyanobacterium which grows on moist soil in Thailand, forming large or massive macroscopic colonies, called thallus, that are consumed as food by local people in the northeast region of Thailand. We have studied the diversity of actinobacteria associated with macroscopic thalli of N. commune using a 16S rRNA gene clone library. A total of 70 clones were sequenced, and 45 clones were found to cluster in ten actinobacterial families, namely, Microbacteriaceae, Dermacoccaceae, Pseudonocardiaceae, Intrasporangiaceae, Micrococcaceae, Solirubrobacteraceae, Corynebacteriaceae, Actinomycetaceae, Mycobacteriaceae, and Micromonosporaceae. The majority of the clones were closely related to Arthrobacter oryzae (5 clones, 11.1 %), Micrococcus endophyticus (4 clones, 8.9 %), and Pseudonocardia ailaonensis (4 clones, 8.9 %). Actinobacterial diversity was high, with a Shannon–Wiener index of 3.25 and a Chao1 index of 187.0 ribotypes with 71.11 % coverage. A total of 57 strains were isolated using 12 different media, and 43 isolates were selected for phylogenetic analysis on the basis of their 16S rRNA gene sequences. The results showed that these isolates belonged to six known genera, including Actinomadura, Arthrobacter, Gordonia, Rhodococcus, Sanguibacter, and Streptomyces. These results suggest that a diverse actinobacterial community is associated with macroscopic thalli of N. commune and that potential novel taxa may exist within this environment.
format Article
author Inthasotti,T.
Pathom-Aree,W.
author_facet Inthasotti,T.
Pathom-Aree,W.
author_sort Inthasotti,T.
title Diversity of actinobacteria associated with Nostoc commune Vaucher ex Bornet & Flahault macrocolonies
title_short Diversity of actinobacteria associated with Nostoc commune Vaucher ex Bornet & Flahault macrocolonies
title_full Diversity of actinobacteria associated with Nostoc commune Vaucher ex Bornet & Flahault macrocolonies
title_fullStr Diversity of actinobacteria associated with Nostoc commune Vaucher ex Bornet & Flahault macrocolonies
title_full_unstemmed Diversity of actinobacteria associated with Nostoc commune Vaucher ex Bornet & Flahault macrocolonies
title_sort diversity of actinobacteria associated with nostoc commune vaucher ex bornet & flahault macrocolonies
publisher Springer Heidelberg
publishDate 2015
url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84924351896&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/38902
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