Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand

Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand

Twenty bacterial strains isolated from the blood of patients with suspected Streptococcus suis infection based on clinical symptoms in northern Thailand between 2009 and 2010 were subjected to phenotypic and genotypic identification. Commercial identification kits and a PCR-based assay targeting the...

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Main Authors: Tien L.H.T., Sugiyama N., Duangsonk K., Tharavichitkul P., Osawa R.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84859018828&partnerID=40&md5=3b2987382f055ec63e750f644e0f8a0a
http://www.ncbi.nlm.nih.gov/pubmed/22446127
http://cmuir.cmu.ac.th/handle/6653943832/3913
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-39132014-08-30T02:35:28Z Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand Tien L.H.T. Sugiyama N. Duangsonk K. Tharavichitkul P. Osawa R. Twenty bacterial strains isolated from the blood of patients with suspected Streptococcus suis infection based on clinical symptoms in northern Thailand between 2009 and 2010 were subjected to phenotypic and genotypic identification. Commercial identification kits and a PCR-based assay targeting the S. suis-specific 16S rDNA sequence correctly identified S. suis isolated from patients in northern Thailand; however, there was a risk of misidentifying S. gallolyticus as S. suis using a PCR assay targeting the S. suis-specific house keeping gene encoding glutamate dehydrogenase. This is the first paper to report S. gallolyticus infection in humans in Thailand. 2014-08-30T02:35:28Z 2014-08-30T02:35:28Z 2012 Article 13446304 22446127 JJIDF http://www.scopus.com/inward/record.url?eid=2-s2.0-84859018828&partnerID=40&md5=3b2987382f055ec63e750f644e0f8a0a http://www.ncbi.nlm.nih.gov/pubmed/22446127 http://cmuir.cmu.ac.th/handle/6653943832/3913 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Twenty bacterial strains isolated from the blood of patients with suspected Streptococcus suis infection based on clinical symptoms in northern Thailand between 2009 and 2010 were subjected to phenotypic and genotypic identification. Commercial identification kits and a PCR-based assay targeting the S. suis-specific 16S rDNA sequence correctly identified S. suis isolated from patients in northern Thailand; however, there was a risk of misidentifying S. gallolyticus as S. suis using a PCR assay targeting the S. suis-specific house keeping gene encoding glutamate dehydrogenase. This is the first paper to report S. gallolyticus infection in humans in Thailand.
format Article
author Tien L.H.T.
Sugiyama N.
Duangsonk K.
Tharavichitkul P.
Osawa R.
spellingShingle Tien L.H.T.
Sugiyama N.
Duangsonk K.
Tharavichitkul P.
Osawa R.
Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand
author_facet Tien L.H.T.
Sugiyama N.
Duangsonk K.
Tharavichitkul P.
Osawa R.
author_sort Tien L.H.T.
title Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand
title_short Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand
title_full Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand
title_fullStr Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand
title_full_unstemmed Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in Northern Thailand
title_sort phenotypic and pcr-based identification of bacterial strains isolated from patients with suspected streptococcus suis infection in northern thailand
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84859018828&partnerID=40&md5=3b2987382f055ec63e750f644e0f8a0a
http://www.ncbi.nlm.nih.gov/pubmed/22446127
http://cmuir.cmu.ac.th/handle/6653943832/3913
_version_ 1681420138329407488

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