Inhibition of capsular protein synthesis of Pasteurella multocida strain P-1059.

A mutant strain, PBA322, was constructed by electroporation of a phagemid containing the coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into the parental strain P-1059 (serovar A:3) of Pasteurella multocida, and the pathogenicity was determined in mice and...

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Main Authors: Sthitmatee,N., Kataoka,Y., Sawada,T.
Format: Article
Published: Japanese Society of Veterinary Science 2015
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http://cmuir.cmu.ac.th/handle/6653943832/39167
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spelling th-cmuir.6653943832-391672015-06-16T08:15:51Z Inhibition of capsular protein synthesis of Pasteurella multocida strain P-1059. Sthitmatee,N. Kataoka,Y. Sawada,T. Veterinary (all) A mutant strain, PBA322, was constructed by electroporation of a phagemid containing the coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into the parental strain P-1059 (serovar A:3) of Pasteurella multocida, and the pathogenicity was determined in mice and chickens. Grayish colonies of the mutant, indicating loss of capsule synthesis, were observed under a stereomicroscope using obliquely transmitted light, while iridescent colonies were observed for the parental strain. Moreover, strain PBA322 showed a low amount of OmpH compared with the parental strain on SDS-PAGE. Additionally, the capsule of strain PBA322 was thinner than that of the parental strain according to electron microscopy, correlating to the attenuation against chickens. In conclusion, strain PBA322, the mutant of P. multocida strain P-1059, was completely attenuated for chickens. 2015-06-16T08:15:51Z 2015-06-16T08:15:51Z 2011-11-01 Article 13477439 2-s2.0-84865829216 21747216 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84865829216&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/39167 Japanese Society of Veterinary Science
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Veterinary (all)
spellingShingle Veterinary (all)
Sthitmatee,N.
Kataoka,Y.
Sawada,T.
Inhibition of capsular protein synthesis of Pasteurella multocida strain P-1059.
description A mutant strain, PBA322, was constructed by electroporation of a phagemid containing the coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into the parental strain P-1059 (serovar A:3) of Pasteurella multocida, and the pathogenicity was determined in mice and chickens. Grayish colonies of the mutant, indicating loss of capsule synthesis, were observed under a stereomicroscope using obliquely transmitted light, while iridescent colonies were observed for the parental strain. Moreover, strain PBA322 showed a low amount of OmpH compared with the parental strain on SDS-PAGE. Additionally, the capsule of strain PBA322 was thinner than that of the parental strain according to electron microscopy, correlating to the attenuation against chickens. In conclusion, strain PBA322, the mutant of P. multocida strain P-1059, was completely attenuated for chickens.
format Article
author Sthitmatee,N.
Kataoka,Y.
Sawada,T.
author_facet Sthitmatee,N.
Kataoka,Y.
Sawada,T.
author_sort Sthitmatee,N.
title Inhibition of capsular protein synthesis of Pasteurella multocida strain P-1059.
title_short Inhibition of capsular protein synthesis of Pasteurella multocida strain P-1059.
title_full Inhibition of capsular protein synthesis of Pasteurella multocida strain P-1059.
title_fullStr Inhibition of capsular protein synthesis of Pasteurella multocida strain P-1059.
title_full_unstemmed Inhibition of capsular protein synthesis of Pasteurella multocida strain P-1059.
title_sort inhibition of capsular protein synthesis of pasteurella multocida strain p-1059.
publisher Japanese Society of Veterinary Science
publishDate 2015
url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84865829216&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/39167
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